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| Relationship between T-bet/GATA-3 ratio in peripheral blood mononuclear cells and tidal breathes lung function and Th1/Th2 cell balance in recurrent wheezing infants |
| CHEN Guohua1 HUANG Weimin2 PAN Zhiwei1 BAI Jun1 |
1.Department of Pediatrics, Foshan Maternal and Child Health Hospital Affiliated to the First Clinical Medical College of Southern Medical University, Guangdong Province, Foshan 518000, China;
2.Department of Neonatal, Nanfang Hospital of the First Clinical Medical College of Southern Medical University, Guangdong Province, Guangzhou 510515, China |
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Abstract Objective To investigate the relationship between T-bet/GATA-3 ratio in peripheral blood mononuclear cells and tidal breathes lung function and helper T lymphocyte (Th) 1/ Th2 cell balance of infants with recurrent wheezing. Methods A total of 120 infants with recurrent wheezing who were followed up in Foshan Maternal and Child Health Hospital Affiliated to the First Clinical Medical College of Southern Medical University from January 2018 to December 2019 as wheezing group and 60 healthy infants with healthy physical examination during the same period as control group. T-bet/GATA-3 ratio, tidal breathes lung function parameters, Th1/Th2 and other indexes between two groups were compared, and the correlation between T-bet/GATA-3 ratio and tidal breathes lung function parameters and Th1/Th2 were analyzed. Results T-bet and T-bet/GATA-3 ratio in wheezing group were significantly lower than those in control group, while GATA-3 was significantly higher than that in control group (P < 0.01). Respiratory rate (RR), tidal breathes peak expiratory flow (PTEF) in wheezing group were significantly higher than those in control group, while the tidal volume (VT), peak time ratio (TPTEF/TE) and peak volume ratio (VPEF/VE) were significantly lower than those in control group (P < 0.01). Th1, Th1/Th2 and γ-interferon (IFN-γ) in wheezing group were significantly lower than those in control group, while Th2 and interleukin-4 (IL-4) were significantly higher than those in control group (P < 0.01). Pearson correlation analysis showed that T-bet/GATA-3 ratio was positively correlated with VT, TPTEF/TE, Th1/Th2 (r = 0.741, 0.567, 0.762, P < 0.01), and negatively correlated with RR (r = -0.717, P < 0.01). Conclusion T-bet/GATA-3 ratio in peripheral blood mononuclear cells of infants with recurrent wheezing is decreased, and it is closely related to the tidal breathes lung function and Th1/Th2 cell-mediated immune imbalance, which may provide a new idea for the diagnosis and treatment of recurrent wheezing infants.
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