加味宫外孕Ⅱ号方对HTR-8/SVneo细胞内质网应激JNK信号通路的凋亡作用

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Abstract Objective To research modified decoction Ⅱ of ectopic pregnancy to nourish the effect of cell apoptosis based on the endoplasmic reticulum stress JNK signaling pathway. Methods Thirty-six female SD rats weighing 180-220 g were divided into six groups by random number table method, each with six rats, namely the negative control group (equal volume of normal saline in the dose of medium traditional Chinese medicine), low, medium and high dose traditional Chinese medicine group (12, 24 and 48 g/[kg·d]), Western medicine group (equal volume of normal saline in the dose of medium traditional Chinese medicine + 7.775 mg/kg Methotrexate), Chinese and Western medicine combination group (24 g/kg+7.775 mg/kg Methotrexate). Blood samples were collected and serum was separated eight days after treatment with traditional Chinese medicine and once after administration of Western medicine. After different groups of drug-containing serum were cultured with HTR-8/SVneo cells for 24 hours respectively, Western blot was used to detect the protein expressions of IRE1α, TRAF2, ASK1, JNK1, JNK2, p-JNK and quantitative real-time PCR (qRT-PCR) was used to detect the mRNA expressions of IRE1α, TRAF2, ASK1, JNK1, JNK2. In addition, cells not treated with serum were used as blank control group to exclude interference caused by complex serum composition. Results Compared with the blank control group, the expression levels of IRE1α, TRAF2, ASK1, JNK1, JNK2 and p-JNK proteins and the expression levels of IRE1α, TRAF2, ASK1, JNK1 and JNK2 mRNA in the negative control group showed no significant changes (P ＞ 0.05). Compared with the negative control group, the expression levels of JNK1 and JNK2 proteins in the traditional Chinese medicine dose groups, the Western medicine group and the Chinese and Western medicine combination group showed no significant changes (P ＞ 0.05), while the expression levels of IRE1α, TRAF2, ASK1, p-JNK proteins, and the expression levels of IRE1α, TRAF2, ASK1, JNK1 and JNK2 mRNA were all increased, with statistically significant differences (all P ＜ 0.05). The expression levels of IRE1α, TRAF2, p-JNK proteins and the expression levels of IRE1α, TRAF2, JNK1 and JNK2 in the high dose traditional Chinese medicine group were higher than those in the low and medium dose traditional Chinese medicine groups, and the differences were statistically significant (all P ＜ 0.05). The expression levels of IRE1α, ASK1, p-JNK proteins and the expression levels IRE1α, ASK1 and JNK1 mRNA in the medium dose traditional Chinese medicine group were higher than those in the low dose traditional Chinese medicine group, and the differences were statistically significant (all P ＜ 0.05). Compared with the high dose traditional Chinese medicine group, the expression levels of IRE1α, ASK1, p-JNK proteins and IRE1α, JNK1 mRNA in the Western medicine group and the Chinese and Western medicine combination group were increased, and the differences were statistically significant (all P ＜ 0.05). Compared with the Western medicine group, the expression levels of IRE1α protein and IRE1α, ASK1, JNK1 and JNK2 mRNA in the Chinese and Western medicine combination group showed no significant changes, and the differences were not statistically significant (P ＞ 0.05). ASK1 protein expression was increased, while TRAF2, p-JNK protein expression and TRAF2 mRNA expression were decreased, with statistically significant differences (all P ＜ 0.05). Conclusion The modified decoction Ⅱ of ectopic pregnancy can cause excessive endoplasmic reticulum stress and activate the JNK signaling pathway, leading to cell apoptosis, and possible relevance dose.

Key words： Endoplasmic reticulum stress JNK signal pathway HTR-8/SVneo cells Modified decoction Ⅱ of ectopic pregnancy Apoptosis

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	JIAN Yongnan1 ZHENG Wenlan2

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JIAN Yongnan1 ZHENG Wenlan2. Effect of modified decoction Ⅱ of ectopic pregnancy on apoptosis of endoplasmic reticulum stress JNK signaling pathway in HTR-8/SVneo cells[J]. 中国医药导报, 2021, 18(3): 4-8,16.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I3/4

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