亲子鉴定案件中21个STR基因座的突变分析

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Abstract Objective To observe and analyze the mutation phenomenon of 21 short tandem repeats STR loci in paternity testing cases, and explore the application value of the multiple amplification typing system in paternity testing. Methods The 1628 samples from 587 paternity test cases were collected from January 2016 to Decomber 2019. The template DNA was extracted by Chelex-100 method, and amplification of 21 STR loci was performed using STRtyper-21-G kit. PCR products were separated and detected by capillary electrophoresis in an ABI 3500 genetic analyzer. Genotyping was done using allelic ladders provided with the kit and the GeneMapperID-X1.3 software. Results Among the 587 cases of paternity testing, 443 were triplets and 114 were doublets; 30 cases (5.11%) were excluded; 557 cases (94.89%) were identified. A total of 20 mutations were observed in 19 mutation cases in 557 cases, including 19 mutations in one step and one mutation in two steps; the ratio of paternal mutation to maternal mutation was 8∶1. Conclusion The 21 STR loci review amplification typing system has high non-paternal exclusion performance, which can meet the needs of daily paternity testing. At the same time, STR locus mutation is a relatively common phenomenon. If necessary, supplement detection of STR locus and the use of sequencing to ensure the accuracy of the identification results.

Key words： Paternity testing Short tandem repeats Mutation

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	ZHOU Bingyi XU Shanshan GU Heng LI Mingzhen ZHENG Lixin▲

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ZHOU Bingyi XU Shanshan GU Heng LI Mingzhen ZHENG Lixin▲. Mutation analysis of 21 STR loci in 587 cases of paternity testing[J]. 中国医药导报, 2021, 18(2): 20-22,43.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I2/20

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