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| Network pharmacology research on active ingredients and mechanism of Jianwei Shoutai Pills in the treatment of spontaneous abortion |
| HE Shan1 NING Yan2 JIANG Shaoyan1 LIU Dayan3 DENG Shaojie1 LIU Xuefang1 ZENG Dan1 |
| 1.Department of Pharmacy, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Guangdong Province, Shenzhen 518028, China; 2.Department of TCM, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Guangdong Province, Shenzhen 518028, China; 3.Department of Genesiology, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Guangdong Province, Shenzhen 518028, China |
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Abstract Objective To screen the main active ingredients of Jianwei Shoutai Pills (JWSTP) based on the network pharmacology technology, predict the targets of the active ingredients and discuss mechanisms of JWSTP in the treatment of spontaneous abortion (SA). Methods The active ingredients and targets in JWSTP were screened on traditional Chinese medicine system pharmacology database and analysis platform. Disease targets of SA were screened and potential targets of JWSTP in the treatment of SA were predicted with GeneCards and OMIM database. The protein-protein interaction of potential targets were constructed on STRING online platform. The gene ontology analysis in biological processes and Kyoto Encyclopedia of Genes and Genomes pathways were enriched by DAVID 6.8 online platform to obtain key pathways, and to construct a network of active ingredients and potential targets and key pathways. Results A total of 18 active ingredients were obtained from JWSTP, and 108 possible targets for the treatment of SA were obtained. The key targets included transcription factor AP-1, tumor necrosis factor, protein kinase AKT1, IL-6, vascular endothelial growth factor A, mitogen-activated protein kinase 1, and so on. The key targets were mainly enriched in biological processes such as positive regulation of transcription from RNA polymerase Ⅱ promoter, apoptosis and inflammatory response, and they are mainly concentrated in PI3K-Akt, mitogen-activated protein kinase, and tumor necrosis factor and other signaling pathways. Conclusion The multi-components, multi-targets and multi-pathways action characteristics of JWSTP were confirmed by network pharmacology technology, and the possible mechanisms of JWSTP in the treatment of SA provided theoretical basis to its mechanisms and active ingredients in further study.
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