原发免疫性血小板减少症患者外周血CD4+T淋巴细胞表面PD-1表达水平分析

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Abstract Objective To detect the expression level of programmed death factor-1 (PD-1) on the surface of CD4+T lymphocytes in peripheral blood of patients with primary immune thrombocytopenia (ITP), and to investigate the role of PD-1 in the pathogenesis of ITP. Methods A total of 100 ITP patients admitted to the Department of Hematology, the First Affiliated Hospital of Hebei North University (hereinafter referred to as “our Hospital”) from December 2015 to December 2017 were selected as the study group. Dexamethasone intravenous infusion of 0.15 mg/ (kg·d) was administered once per day or methylprednisolone intravenous infusion of 0.8 mg/(kg·d) was administered once per day, and prednisolone administration 1 mg/ (kg·d) were given after improvement, and the treatment lasted for four weeks. A total of 50 healthy subjects who underwent physical examination in our outpatient department during the same period were selected as the control group. The expression of PD-1 on the surface of CD4+T lymphocytes in peripheral blood was detected by flow cytometry. The changes of platelet count and PD-1 positive expression rate on the surface of CD4+T lymphocytes before and after treatment in the study group were observed. According to the treatment effect, the study group was divided into the complete response group, the effective group and the invalid group, and the differences of PD-1 positive expression rate on the surface of CD4+T lymphocytes in different treatment groups were compared. Results After treatment, the platelet count in the study group was higher than that before treatment, but still lower than that in the control group, and the differences were statistically significant (all P ＜ 0.05). After treatment, the positive expression rate of PD-1 on the surface of CD4+T lymphocytes in the study group was lower than that before treatment, but still higher than that in the control group, with statistically significant differences (all P ＜ 0.05). After treatment, the positive expression rates of PD-1 on the surface of CD4+T lymphocytes in both the complete response group and the effective group were lower than that before treatment, and the differences were statistically significant (all P ＜ 0.05). The positive expression rate of PD-1 on the surface of CD4+T lymphocytes before and after treatment in the invalid group showed no statistical significance (P ＞ 0.05). The positive expression rate of PD-1 on the surface of CD4+T lymphocytes in the complete response group was lower than that in the effective group and the invalid group, and that in the effective group was lower than that in the invalid group, with statistically significant differences (all P ＜ 0.05). Conclusion The up-regulated expression rate of PD-1 on the surface of CD4+T lymphocytes in peripheral blood may be related to the degree of disease development and prognosis of ITP patients.

Key words： Immune thrombocytopenia Programmed death factor-1 T lymphocytes Cytokines

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	CHEN Zhe1 ZHANG Ling1 LIU Jie1 Zhang Bin2 GAO Binghua1

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CHEN Zhe1 ZHANG Ling1 LIU Jie1 Zhang Bin2 GAO Binghua1. Analysis on expression level of PD-1 on the surface of CD4+T lymphocytes in peripheral blood of patients with primary immune thrombocytopenia[J]. 中国医药导报, 2021, 18(1): 92-95.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I1/92

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