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| Study on the pathogenesis and molecular target of pulpitis |
| Nuerbiyamu·Maimaitiyiming WU Long ZHAO Jin |
| Department of Endodontics, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830000, China |
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Abstract Objective To identify the pathogenesis and molecular target of pulpitis. Methods Differentially expressed genes in pulpitis tissue were identified by GEO data GSE77459 (February 2016) and GSE92681 (December 2017). Enrichment analysis and gene set enrichment analysis were used to evaluate the mechanism of differential genes in pulpitis. The key factors associated with pulpitis were identified by PPI network and molecular experiments. Results There were 1280 differentially expressed genes (DEGs) were identified in pulpitis tissue from GSE77459 data. Eighty-one DEGs were verified by GSE92681 data set. Enrichment analysis and gene set enrichment analysis showed that DEGs was significantly correlated with immune and inflammatory responses. PPI network analysis screened 55 network genes and identified CD44 as the core network factor and regulatory gene. The expression of CD44 in pulpitis tissue was significantly up-regulated by real-time quantitative polymerase chain reaction and Western blot analysis. Conclusion CD44 is a potential biomarker and therapeutic target for pulpitis and is involved in the development of pulpitis through the ERK1/2 signaling pathway.
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