雷公藤红素对人乳腺癌细胞系MDA-MB-231的抑制作用研究

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Abstract Objective To study the antitumor effect of tripterine on MDA-MB-231 breast cancer cells and its mechanism. Methods The effect of tripterine on the growth of MDA-MB-231 was determined by MTT colorimetric method, and its half maximal inhibitory concentration (IC50) was calculated. The experiments on its mechanism were divided into two groups: control group and treatment group. The control group cells were untreated. After the treatment group cells were treated with 2.0 μmol/L tripterine, the ratio of apoptotic cells was determined by Annexin V-FITC fluorescence staining and TUNEL staining. The protein expression levels of p-Akt, Akt, Survivin and Bcl-xL were analyzed by Western blot. Results Tripterine inhibited the growth of MDA-MB-231 with IC50 of 0.93 μmol/L for 48 h. The ratio of Annexin V-FITC staining positive apoptotic cells and the ratio of TUNEL staining positive cells in the treatment group were higher than those in the control group, with statistically significant differences (all P ＜ 0.05). Compared with the control group, p-Akt, Survivin and Bcl-xL protein expressions in the treatment group decreased, and the differences were statistically significant (all P ＜ 0.05). Comparison of Akt total protein expression between the two groups showed no statistically significant difference (P ＞ 0.05). Conclusion Tripterine induces the apoptosis of MDA-MB-231 cells by regulating the Akt signaling pathway, and then inhibits the growth of MDA-MB-231 cells.

Key words： Tripterine Breast cancer Apoptosis Akt signaling pathway

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	SHI Xiaona XU Xia XIE Guie

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SHI Xiaona XU Xia XIE Guie. Inhibitory effect of tripterine on human breast cancer cell line MDA-MB-231[J]. 中国医药导报, 2021, 18(1): 18-21.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I1/18

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