非小细胞肺癌组织中miR-582-5p、Rab27a的表达变化及与临床病理参数的关系

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Abstract Objective To investigate the expression of miR-582-5p and RAS oncogene family Rab27a in non-small cell lung cancer (NSCLC) tissues and their relationship with clinicopathological parameters. Methods The expression of miR-582-5p and Rab27a levels in 120 NSCLC tissues and adjacent tissues were detected by real-time quantitative polymerase chain reaction (qRT-PCR). Rab27a protein expression level in tissues were detected by Western blot. The expressions differences of miR-582-5p and Rab27a and their relationships with clinicopathological features were analyzed statistically. Results Compared with adjacent tissues, the expression level of miR-582-5p was lower than that in NSCLC tissues, while the expression of Rab27a was higher (all P ＜ 0.05). Compared with TNM stage Ⅰ-Ⅱ cancer tissue without lymph node metastasis, the expression level of miR-582-5p in TNM stage Ⅲ-Ⅳ cancer tissue with lymph node metastasis was lower, while the expression level of Rab27a was higher (all P ＜ 0.05). There was a negative correlation between miR-582-5p and Rab27a expression levels in cancer tissues (r = -0.633，P = 0.000). Conclusion The expression level of miR-582-5p is decreased and the expression level of Rab27a is increased in NSCLC tissues, which may be new molecular markers for diagnosis and treatment of NSCLC.

Key words： Non-small cell lung cancer MiR-582-5p Rab27a TNM stage

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	GUO Yi1
	2 GUO Wei2 WANG Yanhong3

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GUO Yi1,2 GUO Wei2 WANG Yanhong3. Expression changes of miR-582-5p and Rab27a in non-small cell lung cancer tissues and their relationship with clinicopathological parameters[J]. 中国医药导报, 2020, 17(35): 108-111.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I35/108

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