温阳散结中药干预乳腺癌细胞SK-BR-3的生长抑制及诱导凋亡研究

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Abstract Objective To research the inhibit proliferation and induce apoptosis effect of Yanghe Huayan Decoction combined with Trastuzumab and PI3K inhibitor (LY294002) on breast cancer cell lines SK-BR-3 (HER-2 overexpression). Methods The intestinal absorption fluid of Yanghe Huayan Decoction was prepared. The breast cancer cell lines SK-BR-3 were divided into blank control group, Chinese medicines group, Trastuzumab group, Trastuzumab + Chinese medicines group, Ly group and Ly + Chinese medicine group. The inhibitory rate of the growth of the cells at 24, 48, 72 h were observed by MTT. The changes of cell apoptosis and cell cycle at 72 h were observed by flow cytometry. Results The inhibition rate, apoptosis rate and S phase cell percentage were increased, while the G2/M phase cell percentage was decreased, in the Chinese medicine group, Trastuzumab group, Trastuzumab + Chinese medicine group, Ly group and the Ly + Chinese medicine group, compared with the blank control group, with statistically significant difference (P < 0.05). Moreover, the inhibition rate of each drug group was increased gradually with the duration of drug action (24, 48, 72 h), presenting a time dependent effect (P < 0.05). The effects of the Trastuzumab group + Chinese medicine, Ly + Chinese medicine group inhibiting cell proliferation, inducing apoptosis and cell cycle arrest were better than those of the Trastuzumab group, Ly group respectively (P < 0.05). Conclusion The intestinal absorption fluid of Yanghe Huayan Decoction can effectively inhibit cell proliferation and induce apoptosis, and the combination of Chinese medicine with Trastuzumab and LY can further inhibit cell proliferation, induce apoptosis, enhance S phase retardation, and inhibit cell entry into G2/M phase. It is suggested that there may be synergistic effect in the treatment of her-2 high expression type of breast cancer by the combination of warming Yang and dispersing masses herbs with Trastuzumab and PI3K inhibitor.

Key words： Warming Yang and dispersing masses therapy Yanghe Huayan Decoction HER-2 overexpressed breast cancer Growth inhibition Cell apoptosis Trastuzumab PI3K inhibitor

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	LIU Xiaofei1 LI Jingwei2 SHI Guangxi2 LI Xiangqi3 ZHANG Yang4 ZHU Qing4

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LIU Xiaofei1 LI Jingwei2 SHI Guangxi2 LI Xiangqi3 ZHANG Yang4 ZHU Qing4. Effect of warming Yang and dispersing masses herbs on growth inhibition and apoptosis induction of breast cancer cell SK-BR-3[J]. 中国医药导报, 2018, 15(2): 13-18.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I2/13

[1] Perou CM，Sorlie T，Eisen MB，et al. Molecular portraits of human breast tumors [J]. Nature，2000，406（6797）：747-753.
[2] 卞磊，陈光伟，马阳.温阳法治疗恶性肿瘤体会[J].实用中医药杂志，2012，28（10）：872-873.
[3] Goldhirsch A，Gelber RD，Piccart-Gebhart MJ，et al. 2 years versus lyear of adjuvant trastuzumab for HER-2 positive breast cancer（HERA）：an open-label randomised controlled trial [J]. Lancet，2013，382（9897）：1021-1028.
[4] 李晓宇，周尔君，顾圣莹，等.荷叶碱大鼠肠道吸收特性研究[J].中国药学杂志，2011，46（18）：1417.
[5] 黄斌，李耿，郭宇飞，等.脑心通胶囊中4个成分肠吸收研究[J].中国中药杂志，2013，38（6）：889.
[6] 江泽，许华，李生水，等，MTT法检测乳腺癌细胞对化疗药物敏感性的研究[J].中国医药导报，2010，3（11）：28-29.
[7] 陈歌，曹阳.温阳化痰散结法治疗乳腺癌之探讨[J].中国中医基础医学杂志2017（6），775-776.
[8] 侯公楷.中医药防止乳腺癌进展[J].辽宁中医药大学学报，2016，5（18），249-253.
[9] 孙慧茹，杨庆有.温阳散结解毒汤的抗肿瘤作用及其对免疫功能影响的实验研究[J].中医研究2010，23（4）：24-28.
[10] 洪日，陈红风，邓樱.复方仙蓉颗粒抑制MCF-10AT乳腺癌癌前病变的研究[J].中华中医药学刊，2010，28（3）：567-570.
[11] 陈歌，安超，斐桂芳，等.温阳化痰散结方对裸鼠乳腺癌移植瘤作用的观察[J].现代中医临床，2016（5）：30-33.
[12] 孙飞，张楚，吴海兰，等.中医药在乳腺癌综合疗法中的地位评价[J].中华中医药杂志，2010，8（12）：1275-128.
[13] 梁颖，徐绍娜，徐放，等．熟地黄多糖对环磷酰胺诱导小鼠的抗突变作用研究[J].中医药信息，2010，27（4）：110-112．
[14] 陈洋洋，王瑞丽，王斌，等.土贝母皂营乙诱导非小细胞肺癌A549细胞凋亡的研究[J].中国中医急症，2017，27（8）：1336-1339.
[15] 徐晓娟，蔡懿鑫，毛宇，等.山慈菇多糖对荷H22肝癌小鼠的抗肿瘤机制研究[J].食品研究与研发，2015，36（7）：23-25.
[16] 赵志梅，张丽杰，夏天，等.莪术主要单体成分抗炎、抗肿瘤作用研究进展[J].药物评价研究，2017，40（1）：199-123.
[17] 姚远，仝立国，冯玛莉，等.黄芪-莪术不同提取物抗肿瘤作用研究[J].中国现代医生，2017，55（7）：33-36.
[18] 刘晓菲，殷玉琨，王蕾，等.阳和化岩汤对大鼠乳腺癌癌前病变的抑制作用研究[J].中华中医药学刊，2014（2）：284-287.
[19] 李静蔚，刘晓菲，陈宏志.温阳散结法干预乳腺癌癌前病变MCF－10AT细胞的生长抑制及诱导凋亡研究[J].中医药学报，2013，41（4）：41-44.
[20] 李静蔚，刘晓菲，梁栋，等.阳和化岩汤对大鼠乳腺癌癌前病变细胞超微结构的影响[J].中医药学报，2014，42（1）：42-46.
[21] 黄斌，陈晓萌，张迎春，等.元胡止痛方肠吸收液对大鼠离体胸主动脉环张力的影响[J].中国实验方剂学杂志，2012，18（5）：117-120.
[22] 陈晓鹏，李昂，褚扬，等.养血清脑颗粒中有效成分的肠外翻吸收研究[J].中国实验方剂学杂志，2013，19（4）：159-162.
[23] Hseu Y C，Chen SC，Chen HC，et al. Antrodia camphorata inhibits proliferation of human breast cancer cells in vitro and in vivo [J]. Food Chem Toxicol，2008，46（8）：2664-2669.