葛根素在脑缺血再灌注损伤时对局部肾素-血管紧张素系统的影响

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Abstract Objective To explore the effect of puerarin on the local cerebrum renin-angiotensin system (RAS) in cerebral ischemia reperfusion injury (IRI). Methods A total of 60 Wistar rats were selected and divided into the control group (sham operation), the model group (rats in the model group were established as cerebral IRI model by inserting a monofilament into middle cerebral artery) and the treatment group (rats in the treatment group were administered with puerarin after the cerebral IRI model was established) by random number table method. All rats were sacrificed 24 h after operation. The neurological function of rats was evaluated by Zea-Longa method. The denatured cell index (DCI) in cerebrum was observed by HE staining. Main components of renin-angiotensin system including angiotensinⅡ (AngⅡ), angiotensin converting enzyme (ACE), angiotensin type 1 receptors (AT1R) and angiotensin type 2 receptors (AT2R) were determined using radioimmunoassay, Real-time PCR and Western blot. Results The neurological function scores, DCI, AngⅡ, ACE, AT1R and AT2R in the model group and the treatment group rats increased significantly compared with those in the control group (P ＜ 0.05). The neurological function scores and DCI after puerarin treatment were decreased significantly compared with those in the model group (P ＜ 0.05), in the meantime, the main components of renin-angiotensin system were also inhibited by puerarin (P ＜ 0.05). Conclusion The protective effect of puerarin on cerebral IRI in rats may have certain relationship to the inhibitory of the local RAS in cerebrum.

Key words： Cerebral ischemia reperfusion injury Puerarin Renin-angiotensin system

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	WANG Ze1 ZHANG Yongtao2 LI Shan1 ZHU Dehui1 JI Xiaojun3

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WANG Ze1 ZHANG Yongtao2 LI Shan1 ZHU Dehui1 JI Xiaojun3. The effect of puerarin on the local cerebrum renin-angiotensin system in cerebral ischemia reperfusion injury[J]. 中国医药导报, 2018, 15(2): 9-12,35.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I2/9

[1] 黄雄峰，汪建民.葛根素的神经保护作用机制研究进展[J].中国实验方剂学杂志，2015，21（4）：224-230.
[2] 黎荣，徐灵源，梁韬，等.葛根素对帕金森病大鼠黑质组织HO-1，NQO1表达的影响[J].中国实验方剂学杂志，2013，19（5）：237-240.
[3] Li R，Zheng N，Liang T，et al. Puerarin attenuates neuronal degeneration and blocks oxidative stress to elicit a neuroprotective effect on substantia nigra injury in 6-OHDA-lesioned rats [J]. Brain Res，2013，15（17）：28-35.
[4] 潘洪平，杨嘉珍，莫祥兰，等.葛根素注射液对急性脑缺血模型大鼠脑细胞损伤的保护作用[J].中国中药杂志，2005，30（6）：457-459.
[5] Zhang Y，Wang L，Song Y，et al. Renin inhibitor aliskiren exerts beneficial effect on trabecular bone by regulating skeletal renin-angiotensin system and kallikrein-kinin system in ovariectomized mice [J]. Osteoporos Int，2016， 27（3）：1083-1092.
[6] 高静媛，杨雨旸，潘彩飞，等.葛根素对糖尿病大鼠心肌保护作用的机制研究[J].中国煤炭工业医学杂志，2017， 20（6）：707-709.
[7] 殷洁，艾琪，刘喆，等.血管紧张素Ⅱ受体拮抗剂类药物对脑神经血管保护机制的研究进展[J].中国脑血管病杂志，2017，14（9）：487-490.
[8] 王婷婷，翟丽，张红艳，等.胡黄连苷Ⅱ对脑缺血损伤大鼠ERK1/2信号通路的影响[J].中国中西医结合杂志，2016， 36（4）：437-444.
[9] Longa EZ，Weinstein PR，Carlson S，et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke，1989，20（1）：84-91.
[10] Nakagawa T，Hasegawa Y，Uekawa K，et al. Transient Mild Cerebral Ischemia Significantly Deteriorated Cognitive Impairment in a Mouse Model of Alzheimer's Disease via Angiotensin AT1 Receptor [J]. Am J Hypertens，2017，30（2）：141-150.
[11] Saavedra JM. Evidence to Consider Angiotensin Ⅱ Receptor Blockers for the Treatment of Early Alzheimer's Disease [J]. Cell Mol Neurobiol，2016，36（2）：259-279.
[12] Zhang TL，Fu JL，Geng Z，et al. The neuroprotective effect of losartan through inhibiting AT1/ASK1/MKK4/JNK3 pathway following cerebral I/R in rat hippocampal CA1 region [J]. CNS Neurosci Ther，2012，18（12）：981-987.
[13] Ishrat T，Pillai B，Soliman S，et al. Low-dose candesartan enhances molecular mediators of neuroplasticity and subsequent functional recovery after ischemic stroke in rats [J]. Mol Neurobiol，2015，51（3）：1542-1553.
[14] 施伟丽，袁蓉，徐浩，等.葛根素防治高血压病的临床与基础研究进展[J].中医药导报，2017，23（12）：105-108.
[15] 吴黎明，江挺，叶榕，等.葛根素预处理抑制NF-κB p65蛋白表达减轻在体大鼠心肌缺血再灌注损伤[J].心血管康复医学杂志，2009，18（2）：185-188.
[16] 郭亚东，邢占良，舒宝瑞，等.葛根素联合替米沙坦对肥胖性高血压患者脂肪因子、肾素-血管紧张素系统活性及胰岛素抵抗的影响[J].河北医药，2016，38（13）：1970-1973.
[17] 周凤华，黄志勇，张蕾，等.葛根素上调miR-155-3p降低内脏脂肪素诱导人脐静脉内皮细胞损伤[J].中国实验动物学报，2016，24（5）：465-469.
[18] Xu XH，Zheng XX，Zhou Q，et al. Inhibition of excitatory amino acid efflux contributes to protective effects of puerarin against cerebral ischemia in rats [J]. Biomed Environ Sci，2007，20（4）：336-342.
[19] Zheng GM，Yu C，Yang Z. Puerarin suppresses production of nitric oxide and inducible nitric oxide synthase in lipopolysaccharide-induced N9 microglial cells through regulating MAPK phosphorylation，O-GlcNAcylation and NF-kappaB translocation [J]. Int J Oncol，2012，40（5）：1610-1618.
[20] Liu X，Mei Z，Qian J，et al. Puerarin partly counteracts the inflammatory response after cerebral ischemia/reperfusion via activating the cholinergic anti-inflammatory pathway [J]. Neural Regen Res，2013，8（34）：3203-3215.
[21] Zhang H，Liu Y，Lao M，et al. Puerarin protects Alzheimer's disease neuronal cybrids from oxidant-stress induced apoptosis by inhibiting pro-death signaling pathways [J]. Exp Gerontol，2011，46（1）：30-37.