Bcl-2和LC3对膀胱癌发生发展及预后的相关性分析

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Abstract Objective To investigate the expression and biological behavior of Bcl-2 and autophagy related gene LC3 in bladder urothelial carcinoma cells, and to explore their effects on the prognosis of bladder cancer. Methods Bcl-2 siRNA plasmid was transfected into human bladder transitional cell carcinoma (T24) cells, Western blot and reverse transcription polymerase chain reaction were used to detect the expression of Bcl-2 and LC-3, and to study Bcl-2 knockout. The effect of T24 on the growth and apoptosis of T24 cells in vitro were studied. The expression of Bcl-2 and LC3 in the pathological specimens of cancer tissues were detected, and the prognosis was analyzed. Results Bcl-2 siRNA recombinant plasmid was successfully transfected into T24 cells, the expression of Bcl-2 mRNA was down-regulated by about 80%, the expression of Bcl-2 protein was down-regulated by about 70%, and the mRNA and protein levels of LC3 were down-regulated by about 40%. The protein expression of Bcl-2 and LC3 in the pGenesil-1 Bcl-2 siRNA group was lower than that of the NC group and the empty plasmid group, and the difference was statistically significant (P ＜ 0.05). The cell viability of T24 decreased to (66.8±5.8) %, and the cell apoptosis rate was 56.68%. The cell viability of the pGenesil-1 Bcl-2 siRNA group were higher than those of the empty plasmid group and the NC group, and the differences were statistically significant (P ＜ 0.05). There was a statistically significant difference in the positive rate of LC3 expression between normal bladder tissue and bladder cancer (P ＜ 0.05). The positive rate of Bcl-2 protein expression in normal bladder tissue and bladder urothelial carcinoma was compared, and the difference was statistically significant (P ＜ 0.05). The expression of LC3 protein in bladder urothelial carcinoma samples of different grades and stages was compared, and the difference was statistically significant (P ＜ 0.05). There was a statistically significant difference in the expression of Bcl-2 protein in bladder urothelial carcinoma samples with or without lymph node metastasis (P ＜ 0.05). Conclusion Down-regulation of Bcl-2 gene will reduce the expression level of bladder cancer cell LC3, inhibit cell growth and promote its apoptosis. The combination of Bcl-2 and LC3 helps to judge the prognosis of bladder urothelial carcinoma.

Key words： Bladder neoplasms Bcl-2 Autophagy-related gene LC3 Autophagy

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	ZHOU Wanli1 ZHANG Jianping1▲ PAN Yongsheng2 XU Weidong1

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ZHOU Wanli1 ZHANG Jianping1▲ PAN Yongsheng2 XU Weidong1. Analysis on the correlation between Bcl-2 and LC3 on the development and prognosis of bladder cancer[J]. 中国医药导报, 2020, 17(34): 43-47.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I34/43

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