不同剂量藏红花提取液对大鼠慢性高眼压模型视神经及轴浆流的影响

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Abstract Objective To investigate the effects of different doses of Stigma Croci extract on optic nerve injury and axoplasma flow in rats with chronic ocular hypertension. Methods A total of 32 SD rats were divided into control group, high ocular hypertension group, low dose group and high dose group by random number table method, with 8 rats and 16 eyes in each group. The bulbar conjunction of rats in the high ocular hypertension group, low dose group and high dose group were cut open, and the cauterized vortex veins were used to make the model of high chronic ocular hypertension, while the bulbar conjunction was only cut open in the control group. The low dose and high dose groups were intraperitoneally injected with 20 mg/kg and 80 mg/kg of Stigma Croci extract respectively and the high ocular hypertension group and the control group were injected with 0.5 mL normal saline for 28 consecutive days. All rats were sacrificed by anesthesia and optic nerves behind the eyeball and retina slices were collected. Ultrathin sections of optic nerve were made and observed under electron microscope. The axoplasma flow and the retinal ganglion cells were observed with countercurrent fluoro-gold. Results The ultrastructure of the optic nerve in the control group was clear, the ultrastructure of the optic nerve in the high ocular hypertension group was seriously damaged, and the ultrastructure of the optic nerve in the low dose group was improved, while the ultrastructure of the optic nerve in the high dose group was significantly improved and close to the control group. Compared with the control group, the number of axons in the high ocular hypertension group decreased, and the difference was highly statistically significant (P ＜ 0.01). Compared with the high ocular hypertension group, the number of axons increased in the low dose group and the high dose group, and the differences were highly statistically significant (all P ＜ 0.01). Compared with the low dose group, the number of axons increased in the high dose group, and the difference was highly statistically significant (P ＜ 0.01). In the control group, the labeled retinal ganglion cells were evenly distributed in all quadrants, and no fluoro-gold leakage was observed outside the cells. Retinal ganglion cells in the high ocular hypertension group had low density in each area and irregular distribution, and some fluoro-gold leakage. The number of retinal ganglion cells increased and individual cells had prominences in the low dose group. In the high dose group, the number of retinal ganglion cells was abundant and there was no leakage and some of the prominences were clearly visible. Compared with the control group, the number of countercurrent fluoro-gold labeled ganglion cells in the high ocular hypertension group decreased, with a highly statistically significant difference (P ＜ 0.01). Compared with the high ocular hypertension group, the number of countercurrent fluoro-gold labeled ganglion cells increased in the low dose group and the high dose group, and the differences were highly statistically significant (all P ＜ 0.01). Compared with the low dose group, the number of countercurrent fluoro-gold labeled ganglion cells increased in the high dose group, and the difference was highly statistically significant (P ＜ 0.01). Conclusion Chronic ocular hypertension can destroy the ultrastructural appearance of rat retrobulbar optic nerve and cut down axoplasm flow; the high dose Stigma Croci extract has a protective effect on the optic nerve and axoplasm flow injured by chronic ocular hypertension.

Key words： Stigma Croci Optic nerve Ultrastructural appearance Axoplasm flow.

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	YU Jingni YANG Xinguang YANG Shangfei GONG Ke WANG Xiaoyu▲

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YU Jingni YANG Xinguang YANG Shangfei GONG Ke WANG Xiaoyu▲. Effects of different doses of Stigma Croci extract on optic nerve and axoplasma flow in rats with chronic ocular hypertension[J]. 中国医药导报, 2020, 17(34): 21-25.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I34/21

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