|
|
|
| Determination on the contents of 6 active ingredients in Glycyrrhizae Radix et Rhizoma by solid phase extraction and high performance liquid chromatography |
| HONG Bo CHEN Gang ZHAO Chaoyang XU Tianjiao SUN Jikai LI Wenjing |
| College of Pharmacy, Qiqihar Medical University, Heilongjiang Province, Qiqihar 161006, China |
|
|
|
Abstract Objective To establish a quantitative method of solid phase extraction and high performance liquid chromatography in determination of six active ingredients in Glycyrrhizae Radix et Rhizoma from different habitats. Methods The effective constituent of Glycyrrhizae Radix et Rhizoma was extracted by solid phase extraction and its active ingredients were determined by high performance liquid chromatography (HPLC). The mobile phase was acetonitrile-0.05% phosphoric acid solution, with gradient elution, flow rate of 1 mL/min, and detection wavelength of 240 nm. Results The six active ingredients albiflorin, paeoniflorin, liquiritin apioside, liquiritin, benzoylpaeoniflorin, glycyrrhizic acid in Glycyrrhizae Radix et Rhizoma showed good linear relationship in the range of 28.8-920.0, 14.7-470.0, 61.9-1980.0, 48.4-1550.0, 71.9-2300.0, 27.5-880.0 μg/mL, the recovery rate was 98.44%, 101.25%, 96.78%, 103.74%, 97.85%, 95.87% respectively, and the RSD was 1.98%, 3.02%, 2.85%, 1.99%, 2.47%, 2.06% respectively. Conclusion Solid phase extraction combined with HPLC in detection of the active ingredients of Glycyrrhizae Radix et Rhizoma is fast and reliable.
|
|
|
|
|
|
[1] Shen SF，Chang ZD，Liu J，et al. Separation of glycyrrhizic acid and liquiritin from Glycyrrhiza uralensis Fisch extract by three-liquid-phase extraction systems [J]. Separation and Purification Technology，2007，53（3）：216-223.
[2] 李冀，李想，曹明明，等.甘草药理作用及药对配伍比例研究进展[J].上海中医药杂志，2019，53（7）：83-87.
[3] Chen J，Zhu XQ，Yang L，et al. Effect of Glycyrrhiza uralensis Fisch polysaccharide on growth performance and immunologic function in mice in Ural City，Xinjiang [J]. Asian Pac J Trop Med，2016，9（11）：1078-1083.
[4] 陈健敏，黄玮玥，叶雅玲，等.甘草提取物的功效及其作用机制研究[J].长江大学学报：自然科学版，2019，16（7）：109-115.
[5] 李倩，高海荣，郭九峰.甘草主要化学成分及药效活性与环境关系研究进展[J].黑龙江农业科学，2019（9）：150-154.
[6] 张欣.复方甘草酸苷对酒精性肝硬化患者肝功能及肝纤维化的影响[J].海峡药学，2018，30（10）：212-213.
[7] Miao XS，Liu RX，Liu HP，et al. Genetic and environmental effect on the growth characteristics and bioactive components of eight-year-old Glycyrrhiza uralensis Fisch [J]. Agri Gene，2017，3：57-62.
[8] Liu YY，Yang YN，Feng ZM，et al. Eight new triterpenoid saponins with antioxidant activity from the roots of Glycyrrhiza uralensis Fisch [J]. Fitoterapia，2019，133：186-192.
[9] 张燕丽，孟凡佳，田园，等.炙甘草的化学成分与药理作用研究进展[J].化学工程师，2019，33（8）：60-63.
[10] Wang WX，Hu XY，Zhao ZY，et al. Antidepressant-like effects of liquiritin and isoliquiritin from Glycyrrhiza uralensis in the forced swimming test and tail suspension test in mice [J]. Prog Neuropsychopharmacol Biol Psychiatry，2008，32（5）：1179-1184.
[11] Chen K，Hu ZM，Song W，et al. Diversity of O-Glycosyltransferases Contributes to the Biosynthesis of Flavonoid and Triterpenoid Glycosides in Glycyrrhiza uralensis [J]. ACS Synth Biol，2019，8（8）：1858-1866.
[12] Zheng ZG，Xu YH，Liu F，et al. Screening bioactive components of Glycyrrhiza uralensis Fisch. with isolated perfused lung extraction and HPLC-ESI-MSn analysis [J]. J Pharm Biomed Anal，2019，169：127-132.
[13] Wang JJ，Chen XQ，Wang W，et al. Glycyrrhizic acid as the antiviral component of Glycyrrhiza uralensis Fisch. against coxsackievirus A16 and enterovirus 71 of hand foot and mouth disease [J]. J Ethnopharmacol，2013，147（12）：114-121.
[14] 任敏霞，吴素香，詹淑玉，等.白芍总苷及其所含主要成分芍药苷和芍药内酯苷对四氯化碳致小鼠急性肝损伤的保护作用[J].中华中医药学刊，2020，38（5）：244-247，283.
[15] 方妍，孙志远，高原，等.甘草炒制前后甘草苷及甘草酸含量的比较[J].长春中医药大学学报，2020，36（3）：485-487.
[16] 章芳珍，吴德玲，赵宏苏，等.苯甲酰芍药苷灌胃给药的代谢产物芍药苷的药动学研究[J].中药新药与临床药理，2019，30（12）：1490-1496.
[17] 郭超峰，施学丽.芍药内酯苷干预抑郁模型大鼠蛋白质组学研究[J].中医学报，2019，34（6）：1248-1252.
[18] 张育贵，张淑娟，边甜甜，等.芍药苷药理作用研究新进展[J].中草药，2019，50（15）：3735-3740.
[19] 刘谦，李熙.采用植物代谢组学技术检测不同产地甘草活性成分的含量[J].亚太传统医药，2019，15（6）：45-49.
[20] Gao YX，Cheng BF，Lian JJ，et al. Liquiritin，a flavone compound from licorice，inhibits IL-1β-induced inflammatory responses in SW982 human synovial cells [J]. Journal of Functional Foods，2017，33：142-148.
[21] Liu ZH，Wang PW，Lu SS，et al. Liquiritin，a novel inhibitor of TRPV1 and TRPA1，protects against LPS-induced acute lung injury [J]. Cell Calcium，2020，88：102198.
[22] 张光辉，郭惠，张拴，等.甘草苷的合成及抗氧化活性研究[J].化学与生物工程，2019，36（10）：16-19.
[23] Nakatani Y，Kobe A，Kuriya M，et al. Neuroprotective effect of liquiritin as an antioxidant via an increase in glucose-6-phosphate dehydrogenase expression on B65 neuroblastoma cells [J]. Eur J Pharmacol，2017，815：381-390.
[24] He SH，Liu HG，Zhou YF，et al. Liquiritin（LT）exhibits suppressive effects against the growth of human cervical cancer cells through activating Caspase-3 in vitro and xenograft mice in vivo [J]. Biomed Pharmacother，2017， 92：215-228.
[25] 周晓雯，鲍真真.苯甲酰芍药苷在大鼠体内的药动学研究[J].江苏中医药，2019，51（2）：80-82.
[26] Sun ZG，Zhao TT，Lu N，et al. Research progress of glycyrrhizic acid on antiviral activity [J]. Mini Rev Med Chem，2019，19（10）：826-832. |
|
|
|
