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| Effect of Coicis Semen oil on intestinal flora and inflammatory factors in mice |
| WANG Tao FEI Jiandong NIE Shuangfa LI Lei |
| Department of Gastrointestinal Surgery, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China |
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Abstract Objective To investigate the effect of Coicis Semen oil on intestinal flora and inflammatory factors. Methods According to the random number table method, 75 SPF male Kunming mice were divided into blank control group, model group, low, medium and high dose group, with 15 mice in each group. Lincomycin was gavaged interfered with the intestinal flora of mice for modeling. After modeling, the model group was given 0.33 g/(kg·d) normal saline, while the low, medium and high dose group were given 0.17, 0.33 and 1.00 g/(kg·d) Coicis Semen oil for 30 days by gavage. The difference of colonic histopathological morphology in each group were observed. The number of Enterococcus, Enterobacter, Bifidobacterium and Lactobacillus, and the weight of mice in each group were compared. The levels of interleukin-6 (IL-6), C-C chemokine ligand 2 (CCL2), IL-1α and IL-1β were compared. Results In the blank control group, the pathological morphology of the colon was normal. In the model group, the inflammation of the pathological morphology was serious. In the low, medium and high dose groups, the pathological morphology was gradually improved compared with the model group. The number of Enterococcus and Enterobacter in the colon of mice in model group were higher than those in blank control group, the number of Bifidobacterium and Lactobacillus in model group were lower than those of the blank control group, and the differences were statistically significant (all P < 0.05). The number of Enterococcus and Enterobacter in the colon of mice in low, medium and high dose groups were lower than those in model group, the number of Bifidobacterium and Lactobacillus in low, medium and high dose groups were higher than those in model group, and the differences were statistically significant (all P < 0.05). On the 30th day, the weight of mice in the low, medium and high dose groups were higher than those in model group, and the differences were statistically significant (all P < 0.05). The levels of IL-6, CCL2, IL-1α and IL-1β in model group were higher than those in the blank control group, while the levels of IL-6, CCL2, IL-1α and IL-1β in the low, medium and high dose groups were lower than those in model group, and the differences were statistically significant (all P < 0.05). Conclusion Coicis Semen oil can improve the imbalance of intestinal flora and inhibit the expression of intestinal inflammatory factors in mice.
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