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| Study on guinea pig model of acute infection with Mycobacterium tuberculosis H37Rv strain |
| KANG Jian1 XU Chengming1 ZHANG Wei2 BAI Yinlan1 HAN Wendong3 SUN Zhiping3 XU Zhikai1 WANG Limei1▲ |
1.Department of Microbiology and Pathogenic Biology, Air Force Military Medical University, Shaanxi Province, Xi′an 710032, China;
2.Department of Pediatric, Tangdu Hospital, Air Force Military Medical University, Shaanxi Province, Xi′an 710032, China;
3.the Third Level Biosafety Laborator, Fudan University, Shanghai 200032, China |
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Abstract Objective To establish a guinea pig model of acute infection with Mycobacterium tuberculosis (Mtb) H37Rv strain. Methods Eighteen guinea pigs were divided into six groups: blank-dose group, subcutaneous high-dose group, subcutaneous low-dose group, intranasal high-dose group, intranasal middle-dose group and intranasal low-dose group according to random number table method. There were three guinea pigs in each group. Subcutaneous high-dose group and subcutaneous low-dose group was infected with Mtb H37Rv PBS suspension of 2×106 CFU/200 μL and 1×106 CFU/200 μL respectively. Intranasal high-dose group, intranasal middle-dose group and intranasal low-dose group was infected with Mtb H37Rv PBS suspension of 5×105, 5×104 CFU/50 μL and 5×103 CFU/50 μL respectively. After four weeks of infection, the number of bacteria and histopathological changes of lung and spleen in each group were detected. Results After four weeks of infection, the number of Mtb in lung and spleen of subcutaneous high-dose group was higher than that of subcutaneous low-dose group (P < 0.05). After four weeks of infection, the number of Mtb in lung of subcutaneous high-dose group was higher than that of intranasal high-dose group (P < 0.05). After four weeks of infection, the lung tissues of guinea pigs in subcutaneous high-dose group, subcutaneous low-dose group and intranasal high-dose group were obviously congested and the alveolar wall were thickened; the lung tissue of guinea pigs in intranasal middle-dose group and intranasal low-dose group had only slight hyperemia. After four weeks of infection, the pathological changes of spleen tissue in each group were not obvious. Conclusion The guinea pig model of acute infection with Mtb H37Rv is established successfully, which provides the research foundation for novel tuberculosis vaccines and new drugs.
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