参草通脉颗粒对慢性心力衰竭大鼠抗心室重构机制的影响

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Abstract Objective To analyze the mechanism of Shencao Tongmai Granules on anti-ventricular remodeling in rats with chronic heart failure (CHF). Methods A total of 60 SPF rats were selected and ten rats were randomly taken as normal group. The other 50 rats were used to prepare the CHF rat model. In the process of modeling, nine rats failed to survive and the remaining 41 rats were divided into the model group (13 rats), the Shencao Tongmai Granules group (14 rats) and the Lisinopril group (14 rats) randomly. Rats in normal group and the model group were fed normally without drugs and rats in Lisinopril group and Shencao Tongmai Granules group were given drugs consecutively for 42 d. Reverse transcription PCR (RT-PCR) and Western blot (WB) were adopted to determine the mRNA and protein expression of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results Compared with the model group, the mRNA and protein expressions of MMP-1 in the Shencao Tongmai Granules group and Lisinopril group were reduced, and the differences were highly statistically significant (all P ＜ 0.01). Compared with the Lisinopril group, the MMP-1 mRNA expression of the Shencao Tongmai Granules group was decreased (P ＜ 0.05), while MMP-1 protein expression was increased (P ＜ 0.05). Compared with the model group, the mRNA and protein expressions of TIMP-1 in the Shencao Tongmai Granules group and Lisinopril group both increased, and the differences were highly statistically significant (all P ＜ 0.01). Compared with the Lisinopril group, the mRNA and protein expressions of TIMP-1 in the Shencao Tongmai Granules group were increased (P ＜ 0.05). Conclusion Shencao Tongmai Granules have a significant therapeutic effect on treating rats with CHF and can promote the mechanism of anti-ventricular remodeling.

Key words： Chronic Heart Failure Shencao Tongmai Granules Matrix metalloproteinase-1 Tissue inhibitor of metalloproteinase-1

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	LIU Xiaolei1 ZHANG Yan2 LI Hai1 WANG Yi1 ZHOU Xue1 CHEN Fei1

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LIU Xiaolei1 ZHANG Yan2 LI Hai1 WANG Yi1 ZHOU Xue1 CHEN Fei1. Effects of Shencao Tongmai Granules on the mechanism of anti-ventricular remodeling in rats with chronic heart failure[J]. 中国医药导报, 2020, 17(30): 8-11.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I30/8

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