潜阳合剂抑制心肌成纤维细胞增殖的机制研究

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Abstract Objective To discussed the mechanism of Qianyang Decoction inhibits the abnormal proliferation of cardiac fibroblasts (RCF) cells induced by angiotensin Ⅱ (Ang Ⅱ). Methods The RCF cells were divided into blank cell group, Ang Ⅱ group and Ang Ⅱ+Qianyang Decoction group. In the Ang Ⅱ group, a cell model with abnormal proliferation of cardiac fibroblasts induced by Ang Ⅱ for 24 h was selected, and the Ang Ⅱ+Qianyang Decoction group was a cell model that was induced by Ang Ⅱ and intervened with Qianyang Decoction. Western blot was used to analyze and compare the transforming growth factor-β1 (TGF-β1), Collagen Ⅰ type (Collagen Ⅰ), Collagen Ⅲ protein expression among three groups, and quantitative polymerase chain reaction was used to detect and compare the TGF-β1 and Collagen Ⅰ, Collagen Ⅲ genetic expression among three groups. Results The protein expression levels of TGF-β1, Collagen Ⅰ and Collagen Ⅲ in Ang Ⅱ group were higher than those in the blank cell group (all P ＜ 0.01). The protein expression levels of TGF-β1 and Collagen Ⅰ, Collagen Ⅲ in Ang Ⅱ+Qianyang Decoction group were lower than those in the Ang Ⅱ group (P ＜ 0.05 or P ＜ 0.01). The gene expressions levels of TGF-β1, Collagen Ⅰ and Collagen Ⅲ in Ang Ⅱ group were higher than those in the blank cell group (all P ＜ 0.01). The gene expression levels of TGF-β1, Collagen Ⅰ and Collagen Ⅲ in Ang Ⅱ+Qianyang Decoction group were lower than those in Ang Ⅱ group (P ＜ 0.05 or P ＜ 0.01). Conclusion Qianyang Decoction can inhibits the abnormal proliferation of RCF cells by reducing the gene and protein expression level of TGF-β1, Collagen Ⅰ and Collagen Ⅲ, hence alleviate heart muscle fibrosis.

Key words： Qianyang Decoction Angiotensin Ⅱ Cardiac fibroblasts Heart muscle fibrosis

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	CHEN Jie RUAN Xiaofen WANG Xiaolong

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CHEN Jie RUAN Xiaofen WANG Xiaolong. Mechanism research of Qianyang Decoction inhibits the proliferation of cardiac fibroblasts[J]. 中国医药导报, 2020, 17(30): 4-7,11.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I30/4

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