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| Mechanistic study of Scutellariae Barbatae Herba in the treatment of pancreatic cancer by using network pharmacology |
| XU Yachen1 XIE Yiqiang2 XIAO Man2 ZHANG Rui2 SUN Zaoxi1 |
1.Department of Hepatobiliary Surgery, the First Affiliated Hospital, Hainan Medical University, Hainan Province, Haikou 570102, China;
2.College of Traditional Chinese Medicine, Hainan Medical University, Hainan Province, Haikou 571199, China |
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Abstract Objective To study the mechanism of action of Scutellariae Barbatae Herba in treating pancreatic cancer by using network pharmacology. Methods Based on TCMSP, NPASS, HIT, TCMID, TCM-ID, CNKI, WANFANG DATA, CQVIP, Science Citation Index (SCI), PubMed databases. The active constituents of Scutellariae Barbatae Herba were searched from June 1971 to October 2019. The prediction platform was used to acquire and screen target genes. Venn analysis was used to screen interaction genes between component target genes and pancreatic cancer specific genes, then determined the key targets of the drug through drug-target-disease cross-linking. Then fully utilized gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) signal pathway enrichment to analyze molecular mechanism of Scutellariae Barbatae Herba in treating the disease. Results A total of 24 kinds of drug-like compounds were obtained, 73 target genes were determined, five core components including baicalein, wogonin, baicalin, quercetin, stigmasterol, and ten key targets such as AKT1, HSP90A1, tumor necrosis factor, ESR1, epidermal growth factor receptor, post-transcriptional gene silencing 2, SRC, matrix metalloproteinase 9, matrix metalloproteinase 2 and AR were screened out. GO enriched in the regulation of apoptosis, inflammation and oxidative stress, and were involved in the specific binding of proteins, ATPases and DNA. KEGG enriched in cancer, pancreatic cancer, cancer proteoglycan, Ras, phosphatidylinositol-3-kinase-protein kinase B pathway. Conclusion Scutellariae Barbatae Herba may regulate multiple signaling pathways through multiple types of targets, affecting multiple function of cells, thus playing a role in the treatment of pancreatic cancer.
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