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Expression of TLR4 in maternal peripheral blood before and after parturition and its relationship with activation of downstream signaling pathways |
CHEN Zixi1* ZHAO Yunzhi2* ZHU Zhaowei1 ZHANG Mengzhe1 LI Xiaoxiao1 XIANG Fenfen1 WU Rong1▲ KANG Xiangdong1▲ |
1.Department of Laboratory Medicine, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;
2.Department of Obstetrics and Gynecology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China |
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Abstract Objective To analyze the expression of Toll-like receptor 4 (TLR4) in maternal peripheral blood before and after parturition and its relationship with activation of downstream signaling pathways. Methods The peripheral blood and uterine muscle tissue of pregnant women admitted to Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from March 2018 to August 2019 were collected and divided into term labor group (21 cases) and term non labor group (20 cases) according to whether or not labor. The expression of TLR4 was detected by flow cytometry and enzyme-linked immunosorbent assay. RT-PCR was used to detect the expression of TLR4, contraction related protein, nuclear factor (NF)-κB, JNK, ERK and P38 mRNA in uterine muscle, and their relationships were analyzed. Results There was no significant difference in age, gestational age and body mass index between two groups (P > 0.05). There were significant differences in the expression levels of peripheral blood neutrophils, monocytes and serum TLR4 between two groups (P < 0.05). There were significant differences in TLR4, CX43 and OTR between two groups (P < 0.05). There were significant differences in the mRNA levels of NF-κB, ERK, P38 and JNK between two groups (P < 0.05). TLR4 was positively correlated with CX43, OTR, JNK, ERK, P38 and NF-κB (R2 = 0.619, 0.416, 0.438, 0.426, 0.55, 0.332, all P < 0.01). JNK, ERK, P38 and NF-κB were positively correlated with CX43 (R2 = 0.548, 0.393, 0.702, 0.600, all P < 0.01), JNK, ERK, P38 and NF-κB were positively correlated with OTR (R2 = 0.308, 0.608, 0.532, 0.514, all P < 0.01). Conclusion TLR4 may play an important role in labor initiation by activating downstream NF-κB/MAPK pathway, promoting the expression of contraction related proteins and promoting uterine contraction.
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[1] You S,Cui AM,Hashmi SF,et al. Dysregulation of bile acids increases the risk for preterm birth in pregnant women [J]. Nat Commun,2020,11(1):2111.
[2] 肖婧.黄体酮联合盐酸利托君治疗先兆早产效果观察[J].中国继续医学教育,2020,12(2):138-141.
[3] 洪秀,盛晓园.早产发生率及高危因素及对围产结局的影响[J].中国医院统计,2019,26(1):218-220.
[4] 平莉莉,张淑彦,翟淑芬.宫内感染早产儿脑损伤与血清炎症因子水平的相关性[J].儿科药学杂志,2019,1(8):4-6.
[5] 王鑫,王婵,郑有宁.宫内感染早产儿血清及脑脊液炎性因子与脑损伤的关系[J].临床和实验医学杂志,2019,18(3):290-292.
[6] 熊小菊,黄鹤.Toll样受体4与心血管疾病研究进展[J].中国心血管病研究杂志,2016,14(12):1061-1064.
[7] 于莉,潘靖丹,杜娈英,等.TLR2和TLR4在抗病毒天然免疫应答中的新作用[J].病毒学报,2018,12(4):570-578.
[8] 吴疑,匡梅,周红,等.TLR4信号通路及其负调控研究进展[J].现代医药卫生,2016,3(18):2821-2824.
[9] 尹姝珂,李卫红.子宫内膜异位症相关细胞因子研究进展[J].大众科技,2017,19(5):90-92.
[10] 孟楠,张思亚,黄琳,等.母胎界面微环境改变与早产[J].中国实用妇科与产科杂志,2018,34(2):135-140.
[11] 黄坚,王珏,林珍云,等.Toll样受体2、3、4、9在胎盘组织中的表达及其与自发性早产的关系[J].浙江医学,2014, 36(7):553-556.
[12] 张艺凡,李星,陈自喜,等.早产和足月产妊娠组织中Toll样受体表达水平及其下游信号通路激活的研究[J].医学临床专集,2017,2017:312-313.
[13] 李乐赛,周昌菊.子宫平滑肌收缩调控机制与分娩启动[J].中国妇幼健康研究,2011,22(5):695-697.
[14] 朱学农,谭玉文,张玉梅,等.Toll样受体在先天性免疫反应中的作用[J].免疫学杂志,2006,22(3):1-3.
[15] Sarah A,Robertson,Hanan H,et al. Toll-like receptor 4:a new target for preterm labour pharmacotherapies? [J]. Current Pharmaceutical Design,2018,24(9):960-973.
[16] 曹耀辉.Toll受体4的表达与胎膜早破患者绒毛膜羊膜炎的关系[J].河北医药,2011,33(4):565-566.
[17] 李爱玲,修瑞娟.Toll样受体4与原发性高血压的相关研究[J].医学综述,2014,20(11):1937-1939.
[18] 陈昱晓,王娜,徐李玲,等.金桔精油通过抑制p38 MAPK和NF-κB信号通路减轻脂多糖诱导的小鼠急性肺损伤[J].广西医科大学学报,2019,36(6):863-868.
[19] 陈湘玲,毕颖.产前血清微量元素含量与产后出血、MAPK信号分子和PGs表达、氧化应激的关系[J].医学院学报,2017,23(7):928-931.
[20] 余镇河,朱昭琼.TLR4参与的炎症通路在术后认知功能障碍中的研究进展[J].遵义医科大学学报,2019,42(6):722-726. |
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