CXCL-13通过激活PI3K-Akt信号通路对人骨髓间充质干细胞迁移的影响

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Abstract Objective To verify C-X-C motif chemokine ligand-13 (CXCL-13) effect on human bone marrow mesenchymal stem cells (hBMSC) migration by activating PI3K-Akt signaling pathway. Methods hBMSC without stimulation was control group. hBMSCs were stimulated with 80 μmol/L CXCL-13 as CXCL-13 group. hBMSCs were first cultured with 25 nmol/L LY294002 for 40 min, and then 80 μmol/L CXCL-13 were added as PI3K inhibitor group. hBMSCs were cultured with 50 μmol/L Triciribine for 30 min in advance and then 80 μmol/L CXCL-13 were added as Akt inhibitor group. Cell wound scratch assay and Transwell cell migration assay were used to detect the scratch area closure rate and cell migration rate of hBMSCs in four groups. The expression of human PI3K, Akt and P-Akt proteins in hBMSCs of four groups were detected by enzyme linked immunosorbent assay. Results The hBMSCs scratch area closure rate and cell migration rate in CXCL-13 group were higher than those in control group, the hBMSCs scratch area closure rate and cell migration rate in PI3K inhibitor group and Akt inhibitor group were lower than those in CXCL-13 group (P ＜ 0.05 or P ＜ 0.01). The protein contents of human PI3K, Akt and P-Akt in CXCL-13 group were higher than those in control group, the protein contents of human PI3K, Akt and P-Akt in PI3K inhibitor group and Akt inhibitor group were lower than those in CXCL-13 group(all P ＜ 0.01). Conclusion CXCL-13 activates the PI3K-Akt signaling pathway to promote the migration of hBMSCs.

Key words： C-X-C motif chemokine ligand-13 Human bone marrow mesenchymal stem cell Akt signaling pathway Cell migration Homing

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	SHEN Lei LI Yongtao SUN Shizhu YAO Lijie WANG Lulu LIU Danyang JIN Haifeng ZHANG Shanqiang

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SHEN Lei LI Yongtao SUN Shizhu YAO Lijie WANG Lulu LIU Danyang JIN Haifeng ZHANG Shanqiang. Effect of CXCL-13 on human bone marrow mesenchymal mtem cell migration by activating PI3K-Akt signaling pathway[J]. 中国医药导报, 2020, 17(27): 4-7.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I27/4

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