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| Effect of Ampelopsin on the differentiation of Treg/Th17 cells in rats with experimental colitis |
| WANG Chao1 WANG Xiaojuan1 ZHU Meirong2 WAN Yugang3 |
1.Department of Internal Medicine, Western Area of Ji′nan Central Hospital CNHTC Hospital, Shandong Province, Ji′nan 250031, China;
2.Department of Surgery, Western Area of Ji′nan Central Hospital CNHTC Hospital, Shandong Province, Ji′nan 250031, China |
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Abstract Objective To investigate the effect of Ampelopsin on the differentiation of Treg/Th17 cells in experimental colitis rats. Methods Forty-eight male SD rats were divided into control group, model group, high dose group, medium dose group, low dose group and Sulfasalazine group by random number table method, with eight rats in each groups. Except for control group, the other five groups of rats were used Trinitrobenzene Sulfonic Scid to establish experimental colitis model. After three days of modeling, drug treatment was given for seven days. Rats in high, medium, and low dose groups were given 500, 250 and 125 mg/(kg·d) of Ampelopsin respectively, and Sulfasalazine group was given 100 mg/(kg·d) of Sulfasalazine, and control group was given quantitative normal saline. The levels of transformed growth factor-β1 (TGF-β1), interleukin-10 (IL-10), IL-17, forkhead box P3 (Foxp3), signal transducers and activators of transcription 5 (STAT5), retinoic related orphan receptor γt (RORγt), STAT3 and IL-35 in six groups were compared. Results The pathological injury score of model group was higher than that of control group (P < 0.05); and the pathological injury scores of high, medium dose groups and Sulfasalazine group were lower than those of model group (all P < 0.05). The expression levels of TGF-β1, IL-10, IL-35, Foxp3 and STAT5 of model group were lower than those of control group, while the expression levels of IL-17, RORγt and STAT3 were higher than those of control group (all P < 0.05). The expression levels of TGF-β1, IL-10, IL-35, Foxp3 and STAT5 of high, medium, low dose groups and Sulfasalazine group were higher than those of model group, while the expression levels of IL-17, RORγt and STAT3 were lower than those of model group (all P < 0.05). Conclusion Ampelopsin can regulate Treg/Th17 cell differentiation and enhance cell function in experimental colitis rats.
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