采用“一测多评”法测定含绿原酸中药及制剂中绿原酸类成分的含量

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Abstract Objective To establish the quantitative analysis of multi-components by single marker (QAMS) method for the analysis of six chlorogenic acids in traditional Chinese medicine and their preparations. Methods A high performance liquid chromatography-photodiode array detector (HPLC-DAD) method was developed with acetonitrile-0.4% phosphoric acid as mobile phase ingradient elution. The column temperature was 25℃. The flow rare was 1.0 mL/min and the detection wavelength was 327 nm. Then the relative correction factors among the other five chlorogenic acids were calculated, when the chlorogenic acid was used as internal referring substances. The ruggedness of relative correction factors was evaluated to establish the QAMS method, when the chlorogenic acid was used as internal referring substances. The content of chlorogenic acid in traditional Chinese medicine (Lonicerae Japonicae Flos and Chrysanthemi Indici Flos) and their eye drops were calculated by the established QAMS method and external standard method respectively, and the content difference of different algorithms was analyzed by t-test and relative error analysis to evaluate the accuracy of the QAMS method. The relative retention time method was used to accurately locate the chromatographic peaks of the six constituents. Results The established relative correction factor with chlorogenic acid as internal referring substances had good ruggedness. Two methods were used to calculate the content of chlorogenic acid in 10 batches of eye drops and three batches of Lonicerae Japonicae Flos and Chrysanthemi Indici Flos. The relative error range was -3.32% to 4.99%, and there was no statistically significant difference (P ＞ 0.05). Conclusion A rapid, accurate and convenient QAMS method for simultaneous measurement of six chlorogenic acids has been established, and this method can be used for quality analysis of six chlorogenic acids in Lonicerae Japonicae Flos, Chrysanthemi Indici Flos and their eye drops.

Key words： Quantitative analysis of multi-components by single marker Relative correction factor Eye drop Lonicerae Japonicae Flos Chrysanthemi Indici Flos

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	ZHANG Qiuyan1 ZHANG Ni1 ZHANG Chunli2 GUO Wenquan2 CUI Hanming1

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ZHANG Qiuyan1 ZHANG Ni1 ZHANG Chunli2 GUO Wenquan2 CUI Hanming1. Content determination of chlorogenic acid in traditional Chinese medicine and their preparations by quantitative analysis of multi-components by single marker[J]. 中国医药导报, 2020, 17(25): 10-14.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I25/10

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