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| In vitro study on the effect of tooth brushing on enamel wear under different oral pH |
| ZHANG Mingshuang1 Ba Te2 ZHAO Qiang2 |
1.College of Stomatology, Weifang Medical University, Shandong Province, Weifang 261021, China;
2.Department of Oral, Aviation Genral Hospital, Beijing 100020, China |
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Abstract Objective To study the dynamic changes of salivary pH and the effect of tooth brushing on enamel wear in different pH environments. Methods From March 10 to 15, 2019, the dynamic changes of saliva pH in 180 medical volunteers in General Hospital at different time points after morning rise were detected for five days (T1: immediately after morning rise, T2:five minutes after morning rise, T3: 10 min after morning rise). From June to September 2019, the isolated teeth collected from the Department of Dental Surgery, Aviation General Hospital were prepared into enamel bases, and after screening, they were divided into two groups: the control group (n = 9) and the experimental group (n = 10), which were soaked with pH=6.8 and 5.8 respectively. The brushing wear experiment was carried out every eight hours. After repeating the experiment for 14 days, the wear depth and surface morphology of the samples were detected. Results There were statistically significant differences in salivary pH values at T1, T2 and T3 after morning rise in 180 healthy subjects (P = 0.01). Compared with T1, saliva pH value increased at T2 and T3, with statistically significant differences (P ＜ 0.05). Compared with T2, salivary pH value increased at T3, with statistically significant difference (P ＜ 0.05). After brushing, the wear depth of both groups increased compared with that before brushing, and the experimental group was higher than the control group, with statistically significant differences (P ＜ 0.05). Scanning electron microscope illustration showed that the surface morphology of the experimental group was coarser. Conclusion Immediately after morning rise, the pH of oral cavity is low and becomes weakly acidic. Brushing in this environment results in increased enamel surface wear. Saliva pH remained stable and close to neutral 10 min after morning rise. In this environment, tooth brushing resulted in less enamel surface wear.
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