|
|
|
| Effect study on MTDH mediated baicalin inhibiting proliferation, migration and invasion of 4T1 breast cancer cells |
| ZHANG Hui1 HUANG Lizhong2 XIAO YuJie2 DENG Tianhao3 GONG Hui3 |
1.Department of Medical Oncology, Chinese Medicine Hospital of Hainan Province, Hainan Province, Haikou 570203, China;
2.College of Combination of Chinese and Western Medicine, Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410007, China;
3.Department of Medical Oncology, Hu′nan Academy of Traditional Chinese Medicine Affiliated Hospital, Hu′nan Province, Changsha 410006, China |
|
|
|
Abstract Objective To investigate the effects of baicalin on proliferation, migration and invasion of 4T1 cells based on metadherin (MTDH) gene. Methods 4T1 cells in logarithmic growth stage were divided into blank control group and baicalin group with different doses of 25, 50, 100, 150 mg/L and 200 mg/L, and then treated with corresponding drugs. Cell activity after baicalin treatment was detected by MTT method. Migration was measured by scratch assay. Invasion was measured by transwell assay and MTDH expression in cells was measured by Western blot. Results The effect of baicalin at 25 mg/L on cell migration was not obvious. As the concentration increased to 50 mg/L, the inhibition of cell migration gradually became obvious. When the concentration increased to 100 mg/L and 150 mg/L, the inhibition of cell migration showed obvious effects. The inhibition rate of cell invasion of baicalin groups with different concentrations was statistically significant (P ＜ 0.05). The inhibition rate of cell invasion in the group of 150 mg/L baicalin was higher than that in the group of 100 mg/L baicalin (P ＜ 0.05). The inhibition rate of cell invasion in the 100 mg/L and 150 mg/L baicalin group was higher than that in the 50 mg/L baicalin group, and the differences were highly statistically significant (P ＜ 0.01). Compared with the control group, MTDH expression was decreased in the 50, 100 mg/L and 150 mg/L baicalin groups (P ＜ 0.05 or P ＜ 0.01). Conclusion Baicalin can inhibit the growth and proliferation of 4T1 breast cancer cells, inhibit the migration and invasion ability of breast cancer cells, and its mechanism may be related to the down-regulation of MTDH expression.
|
|
|
|
|
|
[1] Zhang H，Zhu W，Biskup E，et al. Incidence，risk factors and prognostic characteristics of bone metastases and skeletal-related events（SREs） in breast cancer patients：A systematic review of the real world data [J]. J Bone Oncol，2018，11：38-50.
[2] Nayak D，Katoch A，Sharma D，et al. Indolylkojyl methane analogue IKM5 potentially inhibits invasion of breast cancer cells via attenuation of GRP78 [J]. Breast Cancer Res Treat， 2019，177（2）：307-323.
[3] Yu L，Di Y，Xin L，et al. SND1 acts as a novel gene transcription activator recognizing the conserved Motif domains of Smad promoters，inducing TGFβ1 response and breast cancer metastasis [J]. Oncogene，2017，36（27）：3906-3914.
[4] Juan Xu，Yeying Wang，Zhizheng Li，et al. Ultrasound-Targeted Microbubble Destruction （UTMD） Combined with Liposome Increases the Effectiveness of Suppressing Proliferation，Migration，Invasion，and Epithelial-Mesenchymal Transition（EMT） via Targeting Metadherin （MTDH） by ShRNA [J]. Med Sci Monit，2019，25：2640-2648.
[5] Li T，Li Y，Sun H. MicroRNA-876 is sponged by long noncoding RNA LINC00707 and directly targets metadherin to inhibit breast cancer malignancy [J]. Cancer Manag Res，2019，11：5255-5269.
[6] 王婷，黄立中，肖玉洁，等.黄芩苷联合黄芩素诱导乳腺癌细胞凋亡的机制研究[J].中国现代药物应用，2012，6（9）：10-11.
[7] Wanjun Y，Xingcong M，Xiaoyan G，et al. Research progress in the correlation between baicalein and the invasion and metastasis of breast cancer [J]. Modern Oncology，2016，24（3）：491-494.
[8] Chen X，Li XY，Long M，et al. The FBXW7 tumor suppressor inhibits breast cancer proliferation and promotes apoptosis by targeting MTDH for degradation [J]. Neoplasma，2018，65（2）：201-209.
[9] Wang Zhao，Zheng-Yan Tang，Zhuo Yin，et al. Metadherin regulates epithelial mesenchymal transition in carcinoma [J]. Onco Targets Ther，2016，9：2429-2436.
[10] 李超，宋振川，王新乐，等.异黏蛋白在乳腺癌细胞中的表达[J].中华实验外科杂志，2013，30（12）：62-63.
[11] Liu ZZ，Cao H，Ding YZ，et al. Mechanism research on influence of down-regulating MTDH gene expression in breast canser cell metastasis [J]. Chin J Canser Prev Treat，2014，21（8）：575-579.
[12] Jin PX，Liu ZJ. Expression of metadherin gene in metastatic breast cancer and its clinical significance [J]. Academy Journal of Chinese PLA Medical School，2013，34（2）：133-136.
[13] Dhiman G，Srivastava N，Goyal M，et al. Metadherin：A Therapeutic Target in Multiple Cancers [J]. Front Oncol，2019，9：349.
[14] 冯磊，毛丹，陈州华，等.转移黏附基因在乳腺癌组织中的表达及其与肺转移的相关性研究[J].中南药学，2017， 15（4）：416-420.
[15] 周伟，庾国桢，刘亚敏，等.黄芩苷通过IκBα/NF-κB通路调节Aβ25-35诱导的HT22细胞凋亡[J].中成药，2019，41（11）：2765-2769.
[16] 郑勇凤，王佳婧，傅超美，等.黄芩的化学成分与药理作用研究进展[J].中成药，2016，38（1）：141-147.
[17] 张艳丽，王聪，朱雷蕾，等.黄芩苷药理作用研究进展[J].河南中医.2019，39（9）：1450-1454.
[18] 盂璐，张学武，李正禄.黄芩苷诱导人肝癌HepG-2细胞凋亡及对相关蛋白表达的影响[J].时珍国医国药，2010， 21（9）：2212.
[19] Zhou T，Zhang A. Baicalin inhibits the metastasis of highly aggressive breast cancer cells by reversing epithelial-to-mesen-chymal transition by targeting β-catenin signaling [J]. Oncol Rep，2017，38（6）：3599-3607.
[20] Christofori G. New signals from the invasive front [J]. Nature，2006，441（7092）：444-450. |
|
|
|
