Tim-3/Galectin-9通路、Foxp3在口腔扁平苔藓患者外周血单个核细胞中的表达及意义

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Abstract Objective To study the correlation between Foxp3 and T cell immunoglobulin domain mucin domain protein-3 (Tim-3) and Galactosin -9 (Gal-9) in order to discover the role of Tim-3/Gal-9 pathway in the pathogenesis of oral lichen planus (OLP). Methods From September 2015 to September 2016, 58 OLP patients admitted to the specialized Outpatient Department of Oral Mucosal Disease of the Affiliated Hospital of Guizhou Medical University were selected as the OLP group (36 cases of non-erosive type and 22 cases of hyperemia type). And 17 healthy subjects admitted to the Affiliated Hospital of Guizhou Medical University during the same period were selected as the normal group. The expression levels of Tim-3, Gal-9 and Foxp3 mRNA in peripheral blood mononuclear cells of the two groups were measured by real-time quantitative polymerase chain reaction, and the correlation of the three factors was analyzed. Results The relative expression levels of Tim-3 mRNA and Foxp3 mRNA in non-erosive OLP and hyperemic OLP were higher than those in the normal group (P ＜ 0.05). The relative expression levels of Gal-9 mRNA in all OLP types were not significantly different from those in the normal group (P ＞ 0.05). Foxp3 was positively correlated with Tim-3 and Gal-9 (r = 0.738, P = 0.000; r = 0.443, P = 0.010); there was a positive correlation between Tim-3 and Gal-9 (r = 0.453, P = 0.018). Conclusion Tim-3/Gal-9 signaling mediated Th1 cell apoptosis may be one of the mechanisms by which OLP induces Th2 immune migration, and may be involved in the immune regulation of OLP cells.

Key words： Oral lichen planus T cell immunoglobulin domain mucin domain protein-3 Galectin-9 Foxp3 Real time polymerase chain reaction

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	WANG Dongping1 LUO Liang2 LIU Li3 WANG Xun2 CAI Yang2▲

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WANG Dongping1 LUO Liang2 LIU Li3 WANG Xun2 CAI Yang2▲. The expression and significance of Tim-3/Galectin-9 pathway and Foxp3 in the peripheral blood mononuclear cells of oral lichen planus patients[J]. 中国医药导报, 2020, 17(24): 4-7.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I24/4

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