|
|
|
| Mechanism study of long non-coding RNA ROR1-AS1 promoting osteosarcoma cell invasion |
| ZHANG Yong |
| The First Department of Osteology, Fuxin Traditional Chinese Medicine Hospital, Liaoning Province, Fuxin 123000, China |
|
|
|
Abstract To investigate the effect of lncRNA ROR1-AS1 on the invasion ability of osteosarcoma (OS). Methods RT-PCR detected the expression of lncRNA ROR1-AS1 and ROR1 in osteoblasts and OS cell lines; cell growth was observed after cell transfection, ROR1 was overexpressed, and cell invasion ability was detected by Transwell and scratch test. The expression of epithelial to mesenchymal transformation -related molecular markers (E-caderin, N-caderin, abcam) was detected by Western-blot. Results LncRNA ROR1-AS1 and ROR1 were highly expressed in OS cells compared with osteoblasts (P ＜ 0.01). After lncRNA ROR1-AS1 was inhibited, mRNA expression of ROR1 in OS cells was down-regulated (P ＜ 0.05). After OS cells knocked down lncRNA ROR1-AS1, Transwell and scratch experiments confirmed that the invasion ability of cells was weakened, while the invasion ability was restored after overexpression of ROR1 (P ＜ 0.05). After lncRNA ROR1-AS1 inhibition, the expression of epithelial phenotypic marker E-caderin was decreased, while that of mesenchymal phenotypic marker N-caderin was increased. Overexpression of ROR1 presents the opposite effect. Conclusion LncRNA ROR1-AS1 promotes OS cell invasion dependent on ROR1.
|
|
|
|
|
|
[1] Kager L，Tamamyan G，Bielack S. Novel insights and therapeutic interventions for pediatric osteosarcoma [J]. Future Oncol，2017，13（4）：357-368.
[2] Siegel RL，Miller KD，Jemal A. Cancer statistics，2019 [J]. CA Cancer J Clin，2019，69（1）：7-34.
[3] Oh JY，Kim EH，Lee YJ，et al. Synergistic Autophagy Effect of miR-212-3p in Zoledronic Acid-Treated In Vitro and Orthotopic In Vivo Models and in Patient-Derived Osteosarcoma Cells [J]. Cancers （Basel），2019，11（11）. pii：E1812.
[4] Faisham WI，Mat Saad AZ，Alsaigh LN，et al. Prognostic factors and survival rate of osteosarcoma：A single-institution study [J]. Asia Pac J Clin Oncol，2017，13（2）：e104-e110.
[5] Moreno F，Cacciavillano W，Cipolla M，et al. Childhood osteosarcoma：Incidence and survival in Argentina. Report from the National Pediatric Cancer Registry，ROHA Network 2000-2013 [J]. Pediatr Blood Cancer，2017，64（10）.
[6] Schmitt AM，Chang HY. Long Noncoding RNAs in Cancer Pathways [J]. Cancer Cell，2016，29（4）：452-463.
[7] Bartonicek N，Maag JL，Dinger ME. Long noncoding RNAs in cancer：mechanisms of action and technological advancements [J]. Mol Cancer，2016，15（1）：43.
[8] Qu Z，Li S. Long noncoding RNA LINC01278 favors the progression of osteosarcoma via modulating miR-133a-3p/PTHR1 signaling [J]. J Cell Physiol，2020.
[9] Hou XK，Mao JS. Long noncoding RNA SNHG14 promotes osteosarcoma progression via miR-433-3p/FBXO22 axis [J]. Biochem Biophys Res Commun，2020，523（3）：766-772.
[10] Morris KV，Mattick JS. The rise of regulatory RNA [J]. Nat Rev Genet，2014，15（6）：423-437.
[11] Mattick JS，Rinn JL. Discovery and annotation of long noncoding RNAs [J]. Nat Struct Mol Biol，2015，22（1）：5-7.
[12] Salmena L，Poliseno L，Tay Y，et al. A ceRNA hypothesis：the Rosetta Stone of a hidden RNA language? [J]. Cell，2011，146（3）：353-358.
[13] McAllister SS，Weinberg RA. The tumour-induced systemic environment as a critical regulator of cancer progression and metastasis [J]. Nat Cell Biol，2014，16（8）：717-727.
[14] Nieto MA，Huang RY，Jackson RA，et al. EMT：2016 [J]. Cell，2016，166（1）：21-45.
[15] Oishi I，Takeuchi S，Hashimoto R，et al. Spatio-temporally regulated expression of receptor tyrosine kinases，mRor1，mRor2，during mouse development：implications in development and function of the nervous system [J]. Genes Cells，1999，4（1）：41-56.
[16] Karachaliou N，Gimenez-Capitan A，Drozdowskyj A，et al. ROR1 as a novel therapeutic target for EGFR-mutant non-small-cell lung cancer patients with the EGFR T790M mutation [J]. Transl Lung Cancer Res，2014，3（3）：122-130.
[17] Cui B，Zhang S，Chen L，et al. Targeting ROR1 inhibits epithelial-mesenchymal transition and metastasis [J]. Cancer Res，2013，73（12）：3649-3660.
[18] Dai B，Shen Y，Yan T，et al. Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma [J]. cells，2020，43（2）：601-608.
[19] Hu G，Gupta SK，Troska TP，et al. Long non-coding RNA profile in mantle cell lymphoma identifies a functional lncRNA ROR1-AS1 associated with EZH2/PRC2 complex [J]. Oncotarget，2017，8（46）：80223-80234.
[20] Anastasiadou E，Jacob LS，Slack FJ. Non-coding RNA networks in cancer [J]. Nat Rev Cancer，2018，18（1）：5-18. |
|
|
|
