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| The progress in research methods in molecular biology of nosocomial infection |
| SHAO Yibo GU Youwei LI Xu |
| Department of Infection Management, the First Affiliated Hospital of Anhui Medical University, Anhui Province, Hefei 230022, China |
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Abstract The epidemiological investigation of nosocomial infection pathogens is crucial to establish reasonable infection control measures. In this paper, the progress of molecular biological methods in the epidemiological investigation of nosocomial infection pathogens is described, and the application of some molecular biological methods in the epidemiological investigation of nosocomial infection outbreaks is listed. These molecular typing methods can be categorized into two main groups: DNA-based methods, such as amplified fragment length polymorphism, plasmid fingerprinting, polymerase chain reaction, pulsed field gel electrophoresis and DNA sequencing technology; protein-based methods, such as mutilocus enzyme electrophoresis and immunoblotting. Both kinds of methods have advantages and disadvantages. Therefore, which typing method is employed in practice should be selected based on the situation. It is very important for nosocomial infections to use a reasonable molecular biological typing method.
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