逐水饮治疗恶性胸腔积液大鼠的效果及对其胸膜组织TGF-β1、AQP-1表达的影响

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Abstract Objective To investigate the therapeutic effect of Zhushui Yin on malignant pleural effusion in rats and its effect on expression of transforming growth factor-β1 (TGF-β1) and aquaporin-1 (AQP-1) in rat pleural tissues. Methods The rat model of malignant pleural effusion was established, and the successfully modeled rats were divided into five groups according to the random number table method, namely, the model group (MC), the positive control group (PC), the high-dose group (HD), the middle-dose group (MD), and the low-dose group (LD), with ten rats in each group. Another ten normal rats were recorded as normal group (NC). On the first day after modeling, PC was intraperitoneally injected with 2 mg/kg Cisplatin Injection once every two days. HD, MD and LD were respectively given 66.0, 33.0 g/kg and 16.5 g/kg of Zhushui Yin Liquid once a day. NC and MC were given the same volume of normal saline, once a day, for seven days in total. The amount of pleural effusion and the content of cancer cells in each group were compared. Pleural lesions were observed by hematoxylin-Eosin staining. The TGF-β1 and AQP-1 expression were compared in each group. Results The amount of pleural effusion and the number of cancer cells of MC, PC, HD, MD and LD on the fourth day and the eighth day were all higher than those on the first day, and the eighth day was higher than the fourth day. On the first day, the pleural effusion amount of MC rats was higher than NC, on the fourth day and the eighth day, the pleural effusion amount and cancer cell number of MC rats were higher than those of NC, while PC, HD, MD and LD were lower than MC, HD was lower than PC, LD was higher than PC, and the differences were statistically significant (all P ＜ 0.05). The pleural structure of NC rats was normal. The pleural thickening and infiltration of cancer cells and lymphocytes were serious in MC rats. The pleural thickness in HD, MD, LD was reduced and the infiltration of cancer cells was less. Compared with NC, the expressions of TGF-β1 and AQP-1 in MC were increased (P ＜ 0.05). Compared with MC, the expression levels of TGF-β1 and AQP-1 in PC, HD, MD and LD were all decreased (P ＜ 0.05). Compared with PC, the expression levels of TGF-β1 and AQP-1 in HD were decreased, while the expression levels of TGF-β1 and AQP-1 in LD were increased, with statistically significant differences (all P < 0.05). Conclusion Zhuishui Yin has a good effect on malignant pleural effusion in rats, which may be related to down-regulation of TGF-β1 and AQP-1 expression.

Key words： Zhushui Yin Malignant pleural effusion Transforming growth factor-β1 Aquaporin-1

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	LIU Xiaofang1 WANG Xiaoting2 ZHAO Nan1▲

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LIU Xiaofang1 WANG Xiaoting2 ZHAO Nan1▲. Effect of Zhushui Yin on rats with malignant pleural effusion and its effect on expression of TGF-β1 and AQP-1 in rat pleural tissues[J]. 中国医药导报, 2020, 17(21): 17-21.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I21/17

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