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| Effect of intraocular injection of ozonated saline on rabbit VX-2 tumor by evaluation of ultrasound elastography |
| WANG Lingxiao1 LUO Rong2 JIANG Haibo1 SONG Yushan1 JIN Xiucai1 YANG Chao′ai2 YANG Jijin2 |
1.Department of Ultrasound, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China;
2.Department of Intervention, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China |
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Abstract Objective To evaluate the effect of different times of intraocular injection of ozonized saline on VX-2 tumor in rabbits by ultrasound elastography. Methods A total of 48 New Zealand tumor-bearing white rabbits were randomly divided into four groups by labeling. Among them, 12 rabbits were in the G0 group (injected with low-temperature normal saline, once), while 36 rabbits of the experimental group were given twice the volume of ozonized normal saline under ultrasound guidance, and according to the number of injections, they were divided into G1 (one time), G2 (two times, once a day), G3 group (three times, once a day), with 12 rabbits in each group. The long diameter (a), short diameter (b) and elasticity value (B/A) of the tumor were measured before operation, four and eight days after surgery. The tumor volume (V) was calculated by the formula V=1/2ab2 and tumor growth rate were compared. Results The tumor growth rate of the G1, G3 group four days after surgery were lower than that of the G0 group, and the differences were statistically significant (P ＜ 0.05); the tumor growth rate of G1, G2 and G3 groups were lower than that of the G0 group eight days after surgery, and the differences were statistically significant (P ＜ 0.05). Four days after surgery, the elasticity value of the tumor tissues in the four groups were statistically significant (P ＜ 0.05); among them, the elasticity value of the VX-2 tumor tissues in the G1, G2 and G3 groups were lower than that in the G0 group, and the differences were statistically significant (P ＜ 0.05). Eight days after surgery, the elasticity value of the tumor tissues in the four groups were statistically significant (P ＜ 0.05); among them, the elasticity value of the VX-2 tumor tissues in the G1, G2 and G3 groups were lower than that in the G0 group, while the G2 and G3 groups were lower than that in the G1 group, and G3 group was lower than G2 group, and the differences were statistically significant (P ＜ 0.05). Conclusion Two-dimensional ultrasound combined with elastography can more objectively and comprehensively evaluate the therapeutic effect of different times of intraocular injection of the same concentration of ozonated saline on rabbit VX-2 tumors, and the most obvious decrease in tumor tissue elasticity value after multiple treatments.
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