miR-21/PDCD4/AP-1对食管癌细胞增殖、侵袭及迁移的影响

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Abstract Objective To study the effect of miR-21/PDCD4/AP-1 on the proliferation, invasion and migration of esophageal cancer cells. Methods Transient transfection of esophageal squamous cell carcinoma cells was used as experimental object. The single transfection group: blank control group, miR-21 mimics negative control (miR-21 mimic NC) group, miR-21 mimic group, miR-21 inhibitor NC group, miR-21 inhibitor group, siRNA NC group, PDCD4 siRNA group, c-Jun siRNA group. Cotransfection group: miR-21 inhibitor NC + siRNA NC group, miR-21 inhibitor + siRNA NC group, miR-21 inhibitor + siRNA NC group inhibitor + PDCD4 siRNA group. CCK-8, Transwell migration and invasion were used to detect the effect of miR-21/PDCD4/AP-1 feedback loop on the proliferation, migration and invasion of EC9706 cells. Results From the 2nd day after transfection, the proliferation ability of EC9706 cells in miR-21 mimic group was significantly higher than that in blank control group, miR-21 mimic NC group, miR-21 inhibitor NC group and miR-21 inhibitor group (P < 0.05). The number of cells passing through the chamber in miR-21 mimic group was more than that in blank control group and miR-21 mimic NC group (P < 0.05); the number of cells passing through the chamber in miR-21 inhibitor group was less than that in miR-21 inhibitor NC group (P < 0.05). The number of invasive cells penetrating Matrigel in miR-21 mimic group was more than that in blank control group and miR-21 mimic NC group (P < 0.05); the number of invasive cells penetrating Matrigel in miR-21 inhibitor group was less than that in miR-21 inhibitor NC group (P < 0.05). From the 2nd day after transfection, the proliferation ability of EC9706 cells in PDCD4 siRNA group was higher than that in blank control group and siRNA NC group (P < 0.05). The number of cells passing through the chamber and invading cells passing through Matrigel in PDCD4 siRNA group was higher than that in blank control group and siRNA NC group (P < 0.05). The proliferation ability of EC9706 cells in miR-21 inhibitor + siRNA NC group was lower than that in miR-21 inhibitor + PDCD4 siRNA group and miR-21 inhibitor NC + siRNA NC group (P < 0.05). The number of cells passing through the chamber and the number of cells penetrating Matrigel in miR-21 inhibitor + PDCD4 siRNA group was more than that in miR-21 inhibitor + siRNA NC group (P < 0.05). The proliferation ability of EC9706 cells in c-Jun siRNA group was lower than that in blank control group and siRNA NC group (P < 0.05). The number of cells passing through the chamber and the number of invasive cells passing through Matrigel in c-Jun siRNA group was less than that in siRNA NC group (P < 0.05). Conclusion miR-21/PDCD4/AP-1 feedback loop can regulate the growth and invasion of esophageal cancer cells, which provides a new therapeutic strategy for preventing invasion and metastasis.

Key words： miR-21 PDCD4 AP-1 Esophageal cancer

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	YANG Jingrong1
	2 WU Jian2 YE Shixin2 LIAN Duohuang2 ZENG Zhiyong1
	2▲

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YANG Jingrong1,2 WU Jian2 YE Shixin2 LIAN Duohuang2 ZENG Zhiyong1,2▲. Effect of miR-21/PDCD4/AP-1 on proliferation, invasion and migration of esophageal cancer cells[J]. 中国医药导报, 2020, 17(20): 4-9.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I20/4

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