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| Changes of intestinal flora in patients with ulcerative colitis and its correlation with inflammatory factors and TOLL-like receptor molecules |
| LI Huirong1 CUI Zhao2 LIANG Bo2 LI Guodong3 HE Mingxuan4 LIU Yawei5 ZHOU Jianhua2 |
1.Department of Medical Laboratory, the No.2 Hospital of Baoding, Hebei Province, Baoding 071000, China;
2.Department of Medical Dermatology, the No.2 Hospital of Baoding, Hebei Province, Baoding 071000, China;
3.Department of Gastroenterology, the No.2 Hospital of Baoding, Hebei Province, Baoding 071000, China;
4.Department of Medical Oncology, Baoding First Central Hospital, Hebei Province, Baoding 071000, China;
5.Department of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Hebei University, Hebei Province, Baoding 071000, China |
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Abstract Objective To investigate the changes of intestinal flora in patients with ulcerative colitis (UC) and its correlation with inflammatory factors and TOLL-like receptors (TLRs) molecules. Methods A total of 82 patients with UC admitted to the NO.2 Hospital of Baoding Hebei Province from February 2016 to August 2018 were selected as the observation group, and 70 volunteers who underwent physical examination during the same period were selected as the control group. The levels of interleukin (IL)-4, IL-6, tumor necrosis factor-α (TNF-α), intestinal flora content and the expression of TLRs molecules (TLR4 and TLR9) were detected and compared. Pearson correlation was used to analyze the correlation between TLRs expression and intestinal flora and inflammatory factors. Results The contents of Enterobacter, Bacteroides, Enterococcus and Fusobacterium in the observation group were higher than those in the control group, while the contents of Lactobacillus and Bifidobacterium in the observation group were lower than those in the control group, with statistically significant differences (all P ＜ 0.05). TLR4, TLR9, IL-6 and TNF-α in the observation group were higher than those in the control group, and IL-4 was lower than that in the control group, with statistically significant differences (all P ＜ 0.05). Pearson correlation analysis showed that TLR4 and TLR9 in UC patients were positively correlated with Enterobacter, Bacteroides, Enterococcus, Fusobacterium, IL-6 and TNF-α (r ＞ 0，P ＜ 0.05), but negatively correlated with Lactobacillus, Bifidobacterium and IL-4 (r ＜ 0，P ＜ 0.05). Conclusion In patients with UC, intestinal flora is dysregulated, which can promote the expression of TLRs molecules to increase the level of pro-inflammatory factors, reduce the level of anti-inflammatory factors, and mediate the inflammatory response of intestinal mucosa.
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[1] Ungaro R，Mehandru S，Allen PB，et al. Ulcerative colitis [J]. Lancet，2017，389（10080）：1756-1770.
[2] 李世权，吕晓丹，谢彦飞，等.溃疡性结肠炎小鼠外周血和结肠组织中Tfh、Tfr细胞水平变化及意义[J].山东医药，2018，58（9）：9-12.
[3] 施煜燕，周建波.老年溃疡性结肠炎患者炎症细胞因子、T淋巴细胞亚群和凝血功能变化[J].中国老年学杂志，2018，38（2）：382-384.
[4] Adams SM，Bornemann PH. Ulcerative colitis [J]. Am Fam Physician，2013，87（10）：699-705.
[5] 杨斐，郑晓娟，刘近春，等.溃疡性结肠炎肠道菌群失调的治疗进展[J].胃肠病学，2018，23（1）：56-58.
[6] Ordás I，Eckmann L，Talamini M，et al. Ulcerative colitis [J]. Lancet，2012，380（9853）：1606-1619.
[7] 中华医学会消化病学分会炎症性肠病学组.炎症性肠病诊断与治疗的共识意见（2012年·广州）[J].中华内科杂志，2012，51（10）：818-831.
[8] Cross R，Ko CW，Singh S. Mild-to-Moderate Ulcerative Colitis Guideline [J]. Gastroenterology，2019，156（3）：768.
[9] 王晓宏，张邑.槐花散联合仙方活命饮治疗溃疡性结肠炎临床研究[J].国际中医中药杂志，2017，39（8）：701-704.
[10] Fukuda T，Naganuma M，Kanai T. Current new challenges in the management of ulcerative colitis [J]. Intest Res，2019，17（1）：36-44.
[11] 张永利，申妮.溃疡性结肠炎患者肠道菌群的变化特征[J].河北医药，2018，40（７）：1054-1057.
[12] 刘志威，王学群，李甜甜，等.溃疡性结肠炎患者肠道菌群变化的临床意义[J].胃肠病学和肝病学杂志，2016， 25（5）：554-556.
[13] 李静，陈卫刚.肠道菌群治疗溃疡性结肠炎的研究进展[J].实用医学杂志，2017，33（24）：4189-4192.
[14] 谭芳.益生菌对溃疡性结肠炎大鼠肠黏膜TLRs表达的影响[D].乌鲁木齐：新疆医科大学，2015.
[15] 李顺清.溃疡性结肠炎患者肠道菌群含量以及细胞因子、TLRs分子表达情况的检测[J].海南医学院学报，2017， 23（5）：662-664，668.
[16] 徐晓云，李冬斌，李彬.TLR4、NF-κB p65、IL-8在溃疡性结肠炎中的表达[J].疑难病杂志，2012，11（3）：181-183，封3.
[17] 何雁，王启之.Toll样受体9与溃疡性结肠炎[J].安徽医药，2009，13（6）：593-595.
[18] 陈胜，邹开芳，杨天，等.Toll样受体（TLR）2、TLR4和TLR9在大鼠结肠炎模型结肠组织中的表达及其意义[J].胃肠病学，2007，12（6）：339-343.
[19] Panés J，Alfaro I. New treatment strategies for ulcerative colitis [J]. Expert Rev Clin Immunol，2017，13（10）：963-973.
[20] 岳文杰，刘懿，徐薇，等.溃疡性结肠炎（UC）肠黏膜中IL-2、IL-4、IL-17和IL-10的表达特点及其与疾病活动度的关系[J].复旦学报：医学版，2012，39（5）：454-459，488.
[21] 黄鹤，伦伟健，梁晓燕，等.沙利度胺对溃疡性结肠炎大鼠MMP-9、COX-2、IL-6水平的影响[J].中国老年学杂志，2016，36（7）：1594-1596.
[22] 张爱军，朱叶珊，费亚军等.中药封包电磁波导入辅治溃疡性结肠炎的疗效及对血清炎性因子和免疫球蛋白的影响[J].疑难病杂志，2018，17（4）：370-373.
[23] 邓台燕，全大祥，吴彬，等.复方黄柏液联合美沙拉嗪对溃疡性结肠炎及肠道菌群和血清炎症因子的影响[J].中国现代医学杂志，2016，26（9）：124-127.
[24] 王云滨，陈霞.益生菌联合美沙拉嗪对溃疡性结肠炎患者血清炎症因子及免疫功能的影响[J].中国现代医生，2020，58（9）：40-43.
[25] 李琳，钟青.溃疡性结肠炎患者肠道菌群变化与细胞因子、TLRs分子表达的相关性研究[J].传染病信息，2017， 30（6）：361-364. |
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