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| Expression and function study of kisspeptin/kiss1r system in the ovaries of rats with polycystic ovarian syndrome |
| LIU Ranran1 LUO Qianqian2 SUN Xiaojuan1 LI Lianqin1 ZHANG Hongqin2 |
1.Department of Reproductive Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Shandong Province, Yantai 264100, China;
2.Department of Histology and Embryology, Binzhou Medical University, Shandong Province, Yantai 264003, China |
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Abstract Objective To establish a rat model of polycystic ovary syndrome (PCOS) and investigate the expression and function of kisspeptin/kiss1r system in ovarian tissue. Methods Twenty Wistar rats at the 10th day of gestation were divided into PCOS group and normal group according to the random number table method, with 30 rats in each group. In PCOS group, Letrozole and carboxy methyl cellulose (CMC) were administered continuously for 21 days, while in normal group, CMC of equal volume was administered. Body weight parameters and serum hormone levels of two groups were measured after model establishment; the ovarian histomorphology was detected by HE staining; the expression of kisspeptin and kiss1r in the ovarian tissue was detected by immunohistochemistry and qRT-PCR. Results Compared with normal group, the weight, the content of retroperitoneal fat and the weight of ovary in PCOS group were increased, and the levels of serum luteinizing hormone, follicle stimulating hormone and testosterone were increased (P < 0.05 or P < 0.01). Compared with normal group, the expression of kiss1-mRNA in the ovaries of PCOS group was decreased (P < 0.05). There was no significant difference in kiss1r-mRNA expression between two groups (P > 0.05). PCOS group showed typical polycystic changes in ovaries. Conclusion Down-regulation of kisspeptin/kiss1r system in the ovaries of PCOS rats may play an important role in the occurrence and development of PCOS.
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