靶向MSLN的CAR-NK-92细胞在胃癌中的抗肿瘤活性研究

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Abstract Objective To explore the antitumor activity of NK-92 cells modified with chimeric antigen receptor targeting mesothelin (MSLN) in gastric cancer. Methods The second generation chimeric antigen receptor targeting MSLN was constructed and transfected into NK-92 cells by lentivirus transfection to obtain MSLN CAR-NK-92 cells. The expression of MSLN in human gastric cancer tissues and human gastric cancer cells was detected by immunohistochemistry, flow cytometry and Western blot. The specific recognition ability and the anti-tumor activity of CAR-NK-92 cells were studied through in vitro activation and in vivo tumor inhibition experiments. Results MSLN was highly expressed in human gastric cancer tissue and tumor cells. CAR-NK-92 cells could specifically activate and lyse tumor cells. Compared with NK-92 cells transfected with an empty vector, CAR-NK-92 cells can significantly inhibited the growth of gastric cancer (P < 0.01). Conclusion CAR-NK-92 targeting MSLN has shown significant activity in vivo and in vitro to kill gastric cancer cells specific to MSLN+, which may provide a new idea in the treatment of gastric cancer.

Key words： Gastric cancer Mesothelin NK-92 cell Immunotherapy

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	ZHANG Jun1 DONG Yalu2

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ZHANG Jun1 DONG Yalu2. Study on the antitumor activity of CAR-NK-92 cells targeting MSLN in gastric cancer[J]. 中国医药导报, 2020, 17(19): 11-15.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I19/11

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