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Effect of Acipimox combined with Clopidogrel on the improvement of serum lipids and carotid atherosclerosis in hyperlipidemia rats |
JIANG Aiwen1 DU Peishan2 WANG Shuzhen1 SHI Jinzheng1 LIU Yunning1 LI Fangjiang1 |
1.Department of Medicine, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China;
2.Department of Cardiovascular Medicine, Zhangjiakou First Hospital, Hebei Province, Zhangjiakou 075000, China |
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Abstract Objective To investigate the effect of the improvement of serum lipids and carotid atherosclerosis in hyperlipidemia rats by Acipimox combined with Clopidogrel in a hyperlipidemia rat model. Methods Ten rats from 45 rats were given normal feed as the normal group. The remaining 35 rats were given high-fat feed to model for 4 consecutive weeks, and 5 of them were randomly selected to judge whether the model was successful or not with increased total cholesterol (TC) level and statistically significant difference compared with the normal group. After successful modeling, the remaining 30 rats were randomly divided into the model group, the Acipimox group (32 mg/kg), and the combined group (32 mg/kg Acipimox + 10 mg/kg Clopidogrel ), with 10 rats in each group. The body weight, blood lipid index, hemorheology relevant parameters and serum inflammatory factors of the rats were measured. The carotid artery intimal thickness (IMT) and carotid artery media thickness (MT) were measured by small animal ultrasound. Results The levels of triglyceride (TG), TC and low-density lipoprotein cholesterol (LDL-C) in blood lipid of the Acipimox group were lower than those of the model group, while the levels of high-density lipoprotein cholesterol (HDL-C) were higher than those of the model group, and the differences were statistically significant (all P < 0.05). There was no significant difference in body weight between the two groups (P > 0.05). The levels of TG, TC, and LDL-C in serum lipids in the combined group were significantly lower than those in the model group, while the levels of TC and LDL-C in the combined group were lower than those in the Acipimox group, with statistically significant differences (all P < 0.05). The whole blood viscosity, plasma viscosity and erythrocyte aggregation index in Acipimox group were significantly lower than those in model group, and the differences were statistically significant (all P < 0.05). The whole blood viscosity, plasma viscosity and erythrocyte aggregation index in the combined group were significantly lower than those in the model group, and the whole blood viscosity index (shear rate 5 and 1), plasma viscosity and erythrocyte aggregation index in the combined group were significantly lower than those in the Acipimox group, with statistically significant differences (all P < 0.05). Serum levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the Acipimox group were lower than those in the model group, while IL-10 were higher than those in the model group, and the differences were statistically significant (all P < 0.05). The serum levels of TNF-α and IL-6 in the combined group were lower than those in the model group and the Acipimox group, and the level of IL-10 in the combined group was higher than that in the model group, and the differences were statistically significant (all P < 0.05). IMT and MT in the model group, the Acipimox group and the combined group were all higher than those in the normal group, and the differences were statistically significant (P < 0.05). IMT and MT in Acipimox group were lower than that in model group, and the differences were statistically significant (all P < 0.05). MT in the combined group was significantly lower than that in the model group and the Acipimox group, and IMT was significantly lower than that in the model group, and the differences were statistically significant (all P < 0.05). Conclusion Acipimox can effectively improve the hyperlipidemia and carotid atherosclerosis in rats induced by high-fat diet, and the protective effect is more significant when combined with Clopidogrel.
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