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| Establishment of mice model of inflammation-associated colorectal cancer and expression of vasohibin-1 in colorectal cancer |
| WANG Lei SHI Junli LI Bingqing |
| Department of Gastroenterology, the Affiliated Hospital of Chengde Medical University, Hebei Province, Chengde 067000, China |
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Abstract Objective To investigate the establishment of mice model of inflammation-associated colorectal cancer and expression of vasohibin-1 (VASH-1) in colorectal cancer. Methods Thirty male mice (C57BL/6) were randomly divided into two groups according to the body weight. Group A was given right intraperitoneal administration of azoxymethane (AOM) 10 mg/kg first, and free drink of 1.5%-2.5% dextran sulfate sodium (DSS) at the first, fourth, seventh week later for 7 d, which drunk distilled water on the other time. Group B was given right intraperitoneal administration of normal saline 0.1 mL/10 g, then the mice were given distilled water on the other time. The mice of both groups were killed at the end of tenth week to observe the conditions of tumor formation. The immunohistochemical staining was performed to evaluate the expression of VASH-1 in the of tissue colorectal cancer. Results After modeling, the tumor formation rate of group A was 53.33%, with the pathologic findings of adenocarcinoma, which of group B was 0.00%, the difference was statistically significant (P ＜ 0.05). VASH-1 had high expression in the colorectal cancer and low expression in normal colorectal tissues, with significant difference (P ＜ 0.05). Conclusion The tumor formation rate of mice model of inflammation-associated colorectal cancer induced by this method is high. The expression of VASH-1 in colorectal cancer is higher than that in normal colorectal tissues.
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