病毒性脑炎患儿外周血miR-146a、miR-146b表达检测及临床意义

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Abstract Objective To detect the peripheral blood miR-146a and miR-146b levels in children with viral encephalitis, and to explore their clinical significance. Methods Clinical data of 62 children with viral encephalitis admitted to the First Hospital of Hebei Medical University (“our hospital” for short) from June 2016 to June 2019 and the physical examination data of 43 healthy subjects during the same period in our hospital were retrospectively analyzed, and they were recored as study group and control group respectively. The study group was further divided into 5 subgroups according to the virus type: study group A (7 cases of herpes simplex virus), study group B (9 cases of herpes zoster virus), study group C (28 cases of enterovirus), study group D (6 cases of parotid virus), and study group E (12 cases of epidemic virus B). The levels of miR-146a and miR-146b between the study group, the control group and mild and severe cases of subgroups of study group were compared. The levels of T lymphocyte sub-population were compared between the study group and the control group. The correlations between the levels of miR-146a and miR-146b in peripheral blood and T lymphocyte sub-population levels in study group were analyzed. The levels of miR-146a and miR-146b in peripheral blood of different prognosis were compared. Results The levels of miR-146a and miR-146b in the study group and different sub-groups of the study group were higher than those in the control group (P < 0.05), of which the severe cases were higher than the mild cases (P < 0.05). The CD+3, CD+4 and CD+4/CD+8 in the study group and different sub-groups of the study group were lower than the control group (P < 0.05), while the CD+8 levels were higher than the control group (P < 0.05). The levels of miR-146a and miR-146b of the study group and different sub-groups of the study group were negatively correlated with CD+3, CD+4, and CD+4/CD+8 (P < 0.05), which were positively correlated with CD+8 (P < 0.05). The levels of miR-146a and miR-146b in children with good prognosis were significantly lower than those with poor prognosis (P < 0.05). Conclusion The levels of miR-146a and miR-146b in children with viral encephalitis increase, which can reflect the severity of the conditions and have correlations with the T lymphocyte sub-population, which can predict the prognosis.

Key words： Viral encephalitis miR-146a miR-146b T-lymphocyte sub-population

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	WEI Congzhen ZHAO Xiaojie LI Yanming CHAI Yue BIAN Ce ZHAO Shuai▲

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WEI Congzhen ZHAO Xiaojie LI Yanming CHAI Yue BIAN Ce ZHAO Shuai▲. Detection of peripheral blood miR-146a and miR-146b levels in children with viral encephalitis and their clinical significance[J]. 中国医药导报, 2020, 17(17): 96-100.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I17/96

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