吴茱萸碱对缺氧微环境下胆囊癌细胞增殖及HIF-1α、VEGF表达的影响

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Abstract Objective To investigate the effects of evodotaxine on the proliferation of gallbladder carcinoma cells (CBC-SD) and the expression of hypoxia-inducing factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in GBC-SD in anoxic microenvironment. Methods Using 3%O2 and 5%CO2 to simulate anoxic microenvironment, CBC-SD cells were divided into normal control group, hypoxic group and evodotaxine (100 μg/mL) + hypoxic group, they were cultured in normal and hypoxic microenvironments. MTT assay was used to detect the proliferation of 12, 24, 48, 72 and 96 h cells in each group. Annexin V-FITC/PI double staining flow method was used to detect apoptosis in each group. HIF-1α, VEGF protein expression was detected by Western blot. Results MTT results showed that there was no statistically significant difference in the optical density values between the three groups at 12 h (P ＞ 0.05). At 24, 48, 72 and 96 h, the optical density of the hypoxia group was lower than that of the normal control group, and the difference was statistically significant (P ＜ 0.05). At 24, 48, 72, and 96 h, the light density value of evodotaxine (100 μg/mL) + hypoxia group was lower than that of the hypoxia group, and the difference was statistically significant (P ＜ 0.05). Flow cytometry results showed that the apoptosis rate of hypoxia group was higher than that of normal control group (P ＜ 0.05). The apoptosis rate of evodotaxine (100 μg/mL) + hypoxia group was higher than that of normal control group and hypoxia group, and the difference was statistically significant (all P ＜ 0.05). The protein expression of the HIF-1α, VEGF in the cells of the evodotaxine (100 μg/mL) + anoxic group was lower than that of the normal control group and the hypoxic group, and the differences were statistically significant (all P ＜ 0.05). Conclusion Evodotaxine can inhibit the proliferation of GBC-SD cells in gallbladder cancer under hypoxic microenvironment and promote apoptosis, which may be related to evodotaxine′s inhibition of HIF-1α and VEGF expression in tumor cells.

Key words： Evodiamine Gallbladder carcinoma Hypoxia-inducing factor 1α Vascular endothelial growth factor

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	XU Zhi1 LIU Chunyan2 WEN Juan1 LIU Shaohua3

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XU Zhi1 LIU Chunyan2 WEN Juan1 LIU Shaohua3. Effects of evodotaxine on the proliferation of gallbladder carcinoma cells and the expression of HIF-1α and VEGF in anoxic microenvironment[J]. 中国医药导报, 2020, 17(17): 30-33.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I17/30

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