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| Effect of tetrahydroxystilbene glucoside of Polygonum multiflorum extract on early diabetic nephropathy rats based on TLR4/NF-κB signaling pathway |
| CHEN Hua1 ZHOU Diyi2 |
1.Department of Chinese Medicine, Hangzhou Lin′an Traditional Chinese Medicine Hospital, Zhejiang Province, Hangzhou 310000, China;
2.Department of Endocrinology, Zhejiang Integrated Traditional and Western Medicine Hospital, Zhejiang Province, Hangzhou 310000, China |
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Abstract Objective To explore the effect of Polygonum multiflorum extract on unilateral nephrectomy combined with streptozotocin (STZ)-induced diabetic nephropathy (DN) rats. Methods A total of 60 SPF male Wistar rats were successfully modeled, while divided into control group, model group, irbesartan group, tetrahydroxystilbene glucoside (TSG) low, medium and high dose groups, with 10 rats in each group. Rats were given the corresponding dose of drugs daily, while the control and the model group were infused with a corresponding volume of distilled water. Indexes of rats in each group were recorded and analyzed. Results Compared with the control group, body weight and nuclear factor (NF)-κB inhibition(IκBα) protein expression were reduced in the model group, renal index, the level of serum creatinine (Scr), urea nitrogen (UN), 24 h urine protein content, the levels and mRNA expression of interleukin (IL) -1β, IL-6 and monocyte chemoattractant protein 1(MCP-1), and the protein expression of Toll-like receptor 4 (TLR4), NF-κB p65 and phosphorylated (p)-IκBα were significantly increased, while the glomerular damage was severe, and the differences were highly statistically significant (P ＜ 0.01). Compared with the model group, the weight of each experimental group was significantly increased, the levels of Scr, UN, 24 h urine protein content, IL-1β, IL-6, MCP-1, the protein expression of TLR4, NF-κB p65 and p-IκBα were significantly decreased, while the degree of glomerular damage was significantly improved, and the differences were highly statistically significant (P ＜ 0.01). Conclusion TSG may inhibit the formation of inflammatory factors by down-regulating TLR4/NF-κB signaling pathway to protect the kidney tissue of DN rats.
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