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| Application value analysis of modified CarbaNP method and CIM method in detection of carbapenem-resistant antibiotics Acinetobacter baumannii and Pseudomonas aeruginosa |
| YANG Hua NIU Cui |
| Department of Clinical Laboratory, Xingtai Third Hospital, Hebei Province, Xingtai 054000, China |
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Abstract Objective To investigate the application value analysis of modified CarbaNP method and carbapenems enzyme inactivation method (CIM) in the detection of carbapenem-resistant antibiotics Acinetobacter baumannii and Pseudomonas aeruginosa. Methods A total of 120 strains of Acinetobacter baumannii and 100 strains of Pseudomonas aeruginosa were collected from Xingtai Third Hospital, Hebei Province from January 2014 to June 2015. Bacteria identification and drug susceptibility test were carried out with automatic microbiological instrument. Modified CarbaNP method and CIM method were used to detect bacterial carbapenems, and then gene test was used for validation. Results Among the 120 Acinetobacter baumannii strains, carbapenems antibiotic resistant strains and sensitive strains accounted for 61.67% and 38.33%, respectively. Among the 100 strains of Pseudomonas aeruginosa, carbapenems antibiotic resistant strains and sensitive strains accounted for 60.00% and 40.00% respectively. In Acinetobacter baumannii, the OXA-23 gene carrying rate was 94.59% (70/74), which was taken as the standard, and there was no significant difference between the modified CarbaNP method and the CIM method and the gene detection method (P ＞ 0.05). The modified CarbaNP method and the CIM method were compared with the results of the genetic test respectively which showed that the sensitivity and specificity of the modified CarbaNP method and the CIM method were slightly higher than that of the modified CarbaNP method, but the differences were not statistically significant (P ＞ 0.05). In Pseudomonas aeruginosa, the positive carrying rate of VIM-1 was 93.33% (56/60), which was taken as the standard, and there was no significant difference between the modified CarbaNP method and the CIM method and the gene detection method (P ＞ 0.05). The modified CarbaNP method and the CIM method were compared with the results of the genetic test respectively which showed that the sensitivity and specificity of the modified CarbaNP method and the CIM method were slightly higher than that of the modified CarbaNP method, but the differences were not statistically significant (P ＞ 0.05). Conclusion The modified CarbaNP method and CIM method have some value in the rapid detection of carbapenem-resistant Antibiotics acinetobacter baumannii and Pseudomonas aeruginosa, and the latter has better detection efficiency.
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