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| Effect of the placental pathological changes of murine cytomegalovirus infected mice on the intrauterine growth and development of mice offspring |
| GONG Qi1,2 CHEN Suhua3 MA Chao2 WEI Lei2 ZHANG Zhijun2▲ |
1.Postgraduate Training Base of Jinzhou Medical University, Taihe Hospital of Shiyan City (Affiliated Hospital of Hubei University of Medicine), Hubei Province, Shiyan 442000, China;
2.Center for Reproductive Medicine, Affiliated Hospital of Hubei University of Medicine, Hubei Province, Shiyan 442000, China;
3.Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Hubei Province, Wuhan 430030, China |
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Abstract Objective To observe the efficacy of pathological placental changes after intrauterine murine cytomegalovirus (MCMV) infection on the intrauterine growth and development of fetal mice. Methods The mature mice without MCMV infection identified by enzyme-linked immunoabsorbent assay were served as the subjects. The male and the female mice were put into the same cage. According to the method of random number table, the mice were randomly divided into control group and model group, with 8 mice in each group. At day 12.5 of gestation, the placentas of the model group were injected with MCMV, while the control group was injected with cell maintenance medium. All female mice were executed at day 18.5 of gestation, and the change of tissues and organs of 98 fetal mice were observed. Results The placental infection rate and fetal infection rate in the model group were significantly higher than those in the control group, the differences were statistically significant (P < 0.05). In the placenta infection mice, the fetal infection rate increased significantly compared with those without placenta infection, the difference was statistically significant (P < 0.05). In the placenta infection mice, the rates of stillbirths and abnormal fetuses in mice with cytotrophoblast proliferative, syncytial trophoblast nodules and fibrinoid necrosis were significance higher than those without cytotrophoblast proliferative, syncytial trophoblast nodules and fibrinoid necrosis, the differences were statistically significant (P < 0.05). The teratogenic rate in mice with fibrinoid necrosis was significantly higher than those without fibrinoid necrosis, the difference was statistically significant (P < 0.05). Conclusion The MCMV infection through placenta is an important approach for intrauterine infection. MCMV infection is an important cause of adverse pregnancy outcomes in offspring.
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