铜离子代谢结构域包含体1对胶质瘤细胞增殖与凋亡的作用与机制研究

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Abstract [Abstract] Objective To study the biological function of copper metabolism domain containing 1(COMMD1) in glioma cells U87 and its mechanism. Methods siRNA transfection was applied to knock down COMMD1 expression in U87 cells. CCK-8 and flow cytometry analysis were employed to examine glioma cells proliferation and apoptosis after COMMD1 was knocked down. Western blot was used to detect the expression of p65, cleaveage-caspase3 and BAD levels. Results Data showed that when COMMD1 was knocked down by siCOMMD1, the CCK8 results of siCOMMD1 were (0.402±0.000), (0.510±0.001), (1.021±0.002), (1.612±0.002), while the CCK8 results of siNC were (0.401±0.001), (0.452±0.002), (0.621±0.002), (0.823±0.003) at 0, 1, 2, 3 d; there were significantly differences in cell viabilities between the two group at 2 d and 3 d (P ＜ 0.05). Flow cytometry analysis results showed that the apoptosis rate of cells in siCOMMD1 group and siNC group was (2.214±0.325)% and (7.121±0.520)%, there was significantly difference between the two group (P ＜ 0.05). Western blot results showed that the expression of p65 and BAD increased 40.132% and 90.157%, cleaveage-caspase3 decreased 56.169% when COMMD1 was knocked down. Conclusion COMMD1 can regulate U87 cells apoptosis and proliferation.

Key words： Copper metabolism domain containing 1 Proliferation Apoptosis

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	RUAN Dong1 GUO Lirui2 WANG Fang2 YUAN Fan′en2 CHEN Qianxue2 LIU Baohui2

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RUAN Dong1 GUO Lirui2 WANG Fang2 YUAN Fan′en2 CHEN Qianxue2 LIU Baohui2. The effect of copper metabolism domain containing 1 in U87 cells proliferation and apoptosis and mechanism study[J]. 中国医药导报, 2017, 14(29): 4-6,27.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I29/4

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