小檗碱对大鼠高血压左室肥厚心肌表达miRNA-29b的影响及抑制心肌纤维化的作用机制

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Abstract Objective To investigate the effect of Berberine on expression of miRNA-29b in hypertensive left ventricular hypertrophy myocardial tissue and the mechanism of inhibition for myocardial fibrosis. Methods Male SD rats (n = 25) were randomly divided into sham operation group (sham group, n = 7), model group (model group, n = 9) and model administration group (model+BBR group, n = 9). Rat model of hypertensive cardiac hypertrophy was established by abdominal aortic coarctation. The cardiac structure and function were measured by echocardiography. The cardiac hypertrophy index was measured at 8 weeks postoperatively. The changes of myocardial fibrosis were detected by Masson staining. The expression of miRNA-29b and its target genes col1α1 and col3α1 mRNA in myocardial tissue were detected by RT-PCR. Results IVSd and LVPWd in model group were thicker than those in sham group, and FS and EF were lower than those in Sham group (P < 0.05). IVSd and LVPWd of model+BBR group were thinner than those of model group, and FS and EF were higher than those of model group (P < 0.05). Heart weight of model group was significantly heavier than that of sham group, and heart/body weight ratio was significantly higher than that of sham group (P < 0.01). Heart weight of model+BBR group was lighter than that of model group, and the heart/body weight ratio was lower than that of model group (P < 0.05). The volume fraction of myocardial collagen in model group was significantly higher than that in sham group (P < 0.01). The volume fraction of myocardial collagen in model+BBR group was lower than that in model group (P < 0.05). The level of col1α1 mRNA and col3α1 mRNA in model group was higher than those in sham group, and the level of miRNA-29b was lower than that in sham group (P < 0.05 or P < 0.01). The level of col1α1 mRNA and col3α1 mRNA in model+BBR group was lower than those in model group, and the level of miRNA-29b was higher than that in model group (P < 0.05). Conclusion Berberine inhibits myocardial hypertrophy and myocardial fibrosis by down-regulating miRNA-29b levels and up-regulating its target gene expression.

Key words： Berberine Cardiac hypertrophy Cardiac fibrosis miRNA-29b

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	ZHENG Ying LI Xiaohua LI Qiang ZHANG Yixue CHEN Moshui

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ZHENG Ying LI Xiaohua LI Qiang ZHANG Yixue CHEN Moshui. Effect of Berberine on the expression of miRNA-29b in left ventricular hypertrophy and the mechanism of inhibition of myocardial fibrosis[J]. 中国医药导报, 2020, 17(14): 19-22.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I14/19

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