miRNA-132通过靶向PDCD4在多发性骨髓瘤细胞黏附介导的耐药性中的作用

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Abstract Objective To explore the targeting effect of miR-132 on programmed death 4 gene (PDCD4) and its role in the adhesion mediated drug resistance of multiple myeloma cells. Methods Luciferase reporter gene was used to analyze the targeting regulation of miR-132 on PDCD4. Human myeloma cell line U266 was transfected with miR mimic and inhibitor, and then the drug sensitivity of U266 cells treated with Dexamethasone (Dex), Doxorubicin (Dox) and Bortezomib (Bort) was compared. Transwell method was used to detect the migration ability of U266 cells. MTT and CCK-8 methods were used to detect cell proliferation. Results After Dex and Dox intervention, the inhibition rate of cells increased significantly (P < 0.05), while the inhibition rate of Bort did not change significantly (P > 0.05). Luciferase reporter gene analysis confirmed that PDCD4 was the target gene of miR-132. Up regulation of miR-132 could inhibit the expression of PDCD4 gene and protein (P < 0.05). The migration distance of U266 cells increased (P < 0.05). After miR-132 was up-regulated, the adhesion rate and proliferation activity of U266 cells increased (P < 0.05). Conclusion miR-132 can target PDCD4 to regulate multiple myeloma cell adhesion mediated drug resistance.

Key words： miR-132 Programmed death 4 gene Multiple myeloma Drug resistance Cell adhesion

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	ZHOU Jia′ni1 MA Yongyong2

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ZHOU Jia′ni1 MA Yongyong2. The role of miRNA-132 in multiple myeloma cell adhesion mediated drug resistance by targeting PDCD4[J]. 中国医药导报, 2020, 17(14): 4-7,13.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I14/4

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