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| Effect of 1,25-dihydroxyvitamin D3 delaying renal interstitial fibrosis by T regulatory cell |
| GAO Feng1 GUO Hui2 |
1.The Second Clinical Medical College, Shanxi Medical University, Shanxi Province, Taiyuan 030001, China;
2.Department of Nephrology, the Second Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China |
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Abstract Objective To study the effect of 1, 25-dihydroxyvitamin D3 [1,25(OH)2D3] on renal interstitial fibrosis in rats with unilateral ureteral obstruction (UUO). Methods According to random number table method, 54 wistar rats were divided into sham operation group (group A), UUO group (group B) and 1,25 (OH)2D3 treatment group (group C), with 18 rats in each group. Group C was gavaged 3 ng/(d·100 g) of 1,25（OH）2D3 every day. Group A and group B were gavaged the same amount of peanut oil. The left ureter ligation was performed in group B and group C. Group A was treated with ureteral free, but not ligated. On the 1st, 3rd and 7th day after operation, 6 rats were killed in batches, and the left kidney was retained. Serum creatinine were measured in each group. Renal pathological changes were observed by hematoxylin-eosin (HE) staining. The expression of a-smooth muscle actin (a-SMA) and forkhead P3 protein (FOXP3) in the kidney was detected by immunohistochemistry. Western blot was used to detect the protein expression of phosphorylated adenosine monophosphate activated protein kinase (p-AMPK). Results On the 1st, 3rd and 7th day after operation, the serum creatinine，a-SMA levels of group B was higher than those of group A, and the serum creatinine，a-SMA levels of group C was lower than those of group B (all P < 0.05). The renal tissue morphology of group A was normal, while the renal tissue of group B was prolonged with the obstruction time, showing renal tubules dilatation and interstitial edema, while the tubular degeneration of group C was reduced compared with that of group B. On the 1st, 3rd and 7th day after operation, p-AMPK and FOXP3 levels in group B were lower than those in group A, p-AMPK and FOXP3 levels in group C were higher than those in group B (all P < 0.05). Conclusion 1,25(OH)2D3 can delay renal interstitial fibrosis, and its mechanism may be related to the activation of AMPK to promote the activation of regulatory T cells.
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