糖络宁对糖尿病周围神经病变大鼠背根神经节Toll样受体2/4的影响

Abstract
Figure/Table
References (24)
Related Citation (9)

Download: PDF (503 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the regulating effect of Tangluoning on Toll-like receptors 2/4 (TLR2/4) in dorsal root ganglion (DRG) of rats with diabetic peripheral neuropathy (DPN). Methods Eighty of eight-week-old male SD rats were selected. Fourteen rats were randomly selected as normal group, the rest of 66 rats were intraperitoneally injected with 60 mg/kg streptozotocin to induce DPN rat model, among which 61 rats were succeeded. They were divided into the model group (21 rats), the Western medicine group (19 rats) and the Chinese medicine group (21 rats) by random number table method. Chinese medicine group was gavaged Tangluoning with 5 g/(kg·d), the Western medicine group was gavaged α-lipoic acid with 20 mg/(kg·d), the model group and normal group were gavaged equal amount of distilled water. The drug was administered continuously for 8 weeks. After 8 weeks, the expression of TLR2 and TLR4 proteins in DRG was detected by Western blot. Results The expression levels of TLR2 and TLR4 proteins in normal group were weak, while the expression levels of TLR2 and TLR4 proteins in the model group were higher than those in normal group (P < 0.01). The expression levels of TLR2 and TLR4 proteins in the Chinese medicine group and the Western medicine group were lower than those in the model group (P < 0.05). There were no statistically significant differences in the expression levels of TLR2 and TLR4 proteins between the Chinese medicine group and the Western medicine group (P > 0.05). Conclusion Tangluoning may have impact on the signaling pathways such as the mediated NF-κB by inhibiting the expression of TLR2 and TLR4, thereby reducing inflammation and oxidative stress and reducing nerve damage.

Key words： Tangluoning Toll-like receptor 2/4 Diabetic peripheral neuropathy Inflammation and oxidative stress

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Yixiao1 WANG Liying1 GUO Yuxin1 LIU Qin1 CHEN Feng1 ZHANG Taojing2

Cite this article:

LI Yixiao1 WANG Liying1 GUO Yuxin1 LIU Qin1 CHEN Feng1 ZHANG Taojing2. Effect of Tangluoning on expression of TRL2 /4 in dorsal root ganglion of rats with diabetic peripheral neuropathy[J]. 中国医药导报, 2020, 17(13): 37-40.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I13/37

[1] 朱明瑛，吴坚.糖尿病周围神经病变临床诊断的研究进展[J].医学信息，2019，32（8）：62-66.
[2] Jaiswal M，Divers J，Dabelea D，et al. Prevalence of and Risk Factors for Diabetic Peripheral Neuropathy in Youth With Type 1 and Type 2 Diabetes：SEARCH for Diabetes in Youth Study [J]. Diabetes Care，2017，40（9）：1226-1232.
[3] 高彦彬，周晖，张涛静，等.糖络宁治疗糖尿病周围神经病变临床研究[J].中华中医药杂志，2013，28（6）：1673-1677.
[4] 陈良妹，崔文鹏，罗萍.Toll样受体在1型糖尿病和糖尿病肾病发生发展中作用机制的研究进展[J].吉林大学学报：医学版，2017，43（2）：446-449.
[5] 张延梅，严瑞明，丁玫琳，等.树突状细胞TLR2阻断抑制自身免疫性糖尿病研究[J].中国免疫学杂志，2019，35（8）：902-905.
[6] Kim HS，Han MS，Chung KW，et al. Toll-like receptor 2 senses beta-cell death and contributes to the initiation of autoimmune diabetes [J]. Immunity，2007，27（2）：321-333.
[7] Dasu MR，Park S，Devaraj S，et al. Pioglitazone inhibits Toll-like receptor expression and activity in human monocytes and db/db mice [J]. Endocrinology，2009，150（8）：3457-3464.
[8] 张涛静，龚燕冰，周晖，等.糖络宁对STZ诱导糖尿病大鼠背根神经节线粒体抗氧化剂活性的影响[J].疑难病杂志，2015，14（2）：179-181.
[9] 张涛静，高彦彬，龚燕冰，等.糖络宁对糖尿病大鼠背根神经节线粒体DNA及电镜下线粒体结构的影响[J].中国医药导报，2016，13（11）：4-8.
[10] 李丹，陈龙菊.糖尿病足神经病变动物模型研究进展[J].中国实验动物学报，2018，26（1）：128-132.
[11] 刘琴，李逸潇，张涛静，等.糖络宁对糖尿病大鼠背根神经节线粒体超氧阴离子产生及线粒体膜电位水平的影响[J].中国医药导报，2018，15（26）：4-8.
[12] 郭宇鑫，王利莹，李逸潇，等.糖络宁对糖尿病大鼠周围神经病变氧化应激相关通路的影响[J].北京中医药，2018，37（11）：1018-1021.
[13] 周夏慧，王庆来，朱雪梅，等.当归多糖对DPN大鼠TLR4/MyD88/NF-κB通路抑制影响[J].中国临床药理学与治疗学，2018，23（12）：1340-1347.
[14] 何流，金智生，张磊，等.红芪多糖对糖尿病周围神经病变ob/ob小鼠高迁移率族蛋白1-Toll样受体4信号通路的影响[J].中国临床药理学杂志，2019，35（7）：657-660.
[15] Elzinga S，Murdock BJ，Guo K，et al. Toll-like receptors and inflammation in metabolic neuropathy；a role in early versus late disease? [J]. Exp Neurol，2019，320：112967.
[16] 孙秀菊.α-硫辛酸、甲钴胺、前列地尔联合治疗糖尿病周围神经病变的效果[J].中外医学研究，2019，17（11）：25-27.
[17] Meyers AJ，Shah RR，Gottlieb PA，et al. Altered Toll-like receptor signaling pathways in human type 1 diabetes [J]. J Mol Med（Berl），2010，88（12）：1221-1231.
[18] Zhu T，Meng Q，Ji J，et al. Toll-like receptor 4 and tumor necrosis factor-alpha as diagnostic biomarkers for diabetic peripheral neuropathy [J]. Neurosci Lett，2015， 585：28-32.
[19] Wang YL，Wang K，Yu SJ，et al. Association of the TLR4 signaling pathway in the retina of streptozotocin-induced diabetic rats [J]. Graefes Arch Clin Exp Ophthalmol，2015， 253（3）：389-398.
[20] 王承卉，白洁.Toll样受体4对2型糖尿病及其慢性并发症的影响[J].临床荟萃，2018，33（6）：548-552.
[21] 杨丽梅，贾荣梅，高倩，等.木丹颗粒联合甲钴胺对2型糖尿病周围神经病变气虚络阻证的疗效及对神经电生理和氧化应激指标的影响[J].临床和实验医学杂志，2020，19（2）：181-184.
[22] 王雄，姚春海，刘青云，等.龙牡汤对特应性皮炎小鼠模型的TLR-4、NF-κB p65表达影响[J].中国中西医结合皮肤性病学杂志，2019，18（3）：198-201.
[23] 李芳林，钦卓辉，李辉，等.Toll样受体2激动剂对糖尿病肾损害影响的小鼠实验研究[J].临床和实验医学杂志，2015，14（7）：524-527.
[24] 冯金花.Notch1与TLR2相互作用调控CD14+单核细胞功能在冠状动脉粥样硬化性心脏病中的作用[J].免疫学杂志，2019，35（1）：29-35.