益气活血方治疗心力衰竭的内质网应激机制研究

Abstract
Figure/Table
References (23)
Related Citation (15)

Download: PDF (870 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the molecular mechanism of improvement effect of Yiqi Huoxue Recipe in heart failure treatment by regulating endoplasmic reticulum stress signaling (ERS) pathway. Methods Thirty SPF male SD rats were divided into sham operation group (10 rats) by the random number table method, and the remaining 20 rats model of acute myocardial infarction was prepared by ligating the anterior descending branch of the left coronary artery. Rats with successful modeling were randomly divided into model group and Yiqi Huoxue group, with 10 rats in each group. The rats in model group and sham operation group were gavaged with deionized water 5 mL/(kg·d), 1 time/d. Rats in Yiqi Huoxue group were gavaged with Yiqi Huoxue Recipe 5 mL/(kg·d). The drug was administered continuously for 4 weeks. Hemodynamic index including left ventricular end-diastolic pressure (LVEDP), left ventricular internal pressure maximal rate of increase (+dp/dt max), left heart maximum drop rate of indoor pressure (-dp/dt max), left ventricular systolic pressure (LVSP) and heart rate (HR) were detected by left ventricular intubation method. The expressions of brain natriuretic peptide (BNP) and calcineurin A (CaNA) mRNA were detected by Rt-PCR and the protein levels of caspase-12, protein kinase R-like endoplasmic reticulum kinase (PERK), inositol requiring enzymes 1(IRE1) and glucose-regulated protein 78 (GRP78) were detected by Western blot. Results LVEDP, -dp/dt max and HR in model group were higher than those in sham group, +dp/dt max and LVSP were significantly lower than those in sham operation group, and the differences were highly statistically significant (P < 0.01). LVEDP, -dp/dt max and HR of rats in Yiqi Huoxue group were lower than those in model group, LVSP and +dp/dt max were higher than those in model group, and the differences were highly statistically significant (P < 0.01). The expressions of BNP and CaNA mRNA in model group were higher than those in sham operation group, with statistically significant differences (P < 0.05 or P < 0.01). The expressions of BNP and CaNA mRNA in Yiqi Huoxue group were lower than those in model group, with statistically significant differences (P < 0.05). The expression levels of caspase-12, PERK, IRE1 and GRP78 in model group were higher than those in sham operation group, with statistically significant differences (P < 0.05). The expression levels of caspase-12, PERK and IRE1 in Yiqi Huoxue group were lower than those in model group, with statistically significant differences (P < 0.05). Conclusion Yiqi Huoxue Recipe can improve the cardiac function of rats with heart failure induced by myocardial infarction, and the mechanism included may be related to the endoplasmic reticulum stress signaling pathway regulation.

Key words： Yiqi Huoxue Recipe Endoplasmic reticulum stress Heart failure Cardiac function

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHANG Ting1 WANG Jie2 WU Aiming1 MA Zhe1 ZHAO Yizhou1 ZHANG Dongmei1 JIN Qiushuo1 LOU Lixia1

Cite this article:

ZHANG Ting1 WANG Jie2 WU Aiming1 MA Zhe1 ZHAO Yizhou1 ZHANG Dongmei1 JIN Qiushuo1 LOU Lixia1. Study on endoplasmic reticulum stress mechanism of treating heart failure with Yiqi Huoxue Recipe[J]. 中国医药导报, 2020, 17(13): 32-36,40.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I13/32

[1] 葛均波，徐永健.内科学[M].北京：人民卫生出版社，2014.
[2] 邱伯雍，王永霞.慢性心力衰竭流行病学及防治研究进展[J].中华实用诊断与治疗杂志，2017，31（6）：619-621.
[3] 朱波.李七一心衰辨治心法[J].江苏中医药，2008，40（12）：18-19.
[4] 易建光.益气活血方对早期心衰大鼠心脏重塑影响及心脏细胞形态变化研究[J].四川中医，2018，36（5）：59-62.
[5] 刘先明，熊新忠.益气活血方治疗冠心病心衰30例临床观察[J].中国中医急症，2006，15（9）：946-947.
[6] 王婕，吴爱明，李春红，等.益气活血方对大鼠心肌梗死边缘区Sigma-1R、SERCA2a、IP3R mRNA表达的影响[J].中西医结合心脑血管病杂志，2018，16（9）：1180-1184.
[7] 刘芳，王常林.益气活血方对气虚血瘀型慢性心衰的治疗效果及可能机理[J].中药材，2017，40（1）：230-232.
[8] 娄利霞，唐朝枢.内质网应激与心血管疾病[J].中国分子心脏病学杂志，2005，5（2）：496-499.
[9] 李春红，王婕，吴爱明，等.内质网应激与心力衰竭的相关性研究进展[J].中西医结合心脑血管病杂志，2018，16（12）：1662-1666.
[10] Wu A，Lou L，Zhai J，et al. miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction [J]. Int J Genomics，2017，2017：2175871.
[11] 徐叔云，卞如濂，陈修.《药理实验方法学》第二版出版发行[J].中国药理学通报，1992（1）：19.
[12] 陈丽青.益气活血法施治本虚标实型慢性心力衰竭的临床效果观察[J].心血管防治知识：学术版，2018（4）：59-61.
[13] 郑宇新，孙腾，朱洪.益气活血方联合西药治疗慢性心力衰竭35例观察[J].浙江中医杂志，2011，46（10）：719.
[14] 庄智伟，鲍逸民，杨永青.98例慢性心力衰竭患者血浆BNP和血脂变化及意义[J].放射免疫学杂志，2010，23（3）：337-339.
[15] 王伟涛.监测血清甲状腺激素、Hcy、BNP水平在急性心肌梗死患者中的临床价值[J].中外医学研究，2019， 17（34）：81-83.
[16] 王玉红，刘晖，韩鹏飞，等.脑梗死合并心房颤动患者BNP水平对急性期抗栓治疗效果的影响[J].中国医药科学，2018，8（7）：224-226.
[17] 何俊华.益气活血中药治疗慢性心力衰竭的临床观察[J].内蒙古中医药，2014，33（10）：27-28.
[18] 于桂芝，张玉武，贾志荣，等.益气活血法治疗心衰（气虚血瘀证）的临床研[J].临床医药文献电子杂志，2018， 5（8）：73，76.
[19] 徐多娇，韩秋月，王红霞，等.抑制免疫蛋白酶体亚基β5i性加重小鼠心脏缺血/再灌注损伤[J].中国分子心脏病学志，2017，17（1）：1979-1982.
[20] 陈鹏，杨成明，曾春雨，等.心肌梗死后心力衰竭小鼠心肌组织内质网应激相凋亡途径的研究[J].中国病理生理杂志，2010，26（6）：1069-1074.
[21] 滕旭，齐永芬，唐朝枢.内质网应激与心脏疾病[J].生理科学进展，2009，40（2）：106-110.
[22] Glembotski CC. Endoplasmic reticulum stress in the heart [J]. Circ Res，2007，101（10）：975-984.
[23] Sun Y，Liu G，Song T，et al. Upregulation of GRP78 and caspase-12 in diastolic failing heart [J]. Acta Biochim Pol，2008，55（3）：511-516.