基于Nrf2/HO-1通路研究芹菜素改善妊娠期糖尿病大鼠氧化应激损伤

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Abstract Objective To study the effect of apigenin on oxidative stress injury in gestational diabetic rats based on Nrf2/HO-1 pathway. Methods Thirty-six pregnant SD rats of high-fat feeding were selected, the gestational diabetes rat model was established by intraperitoneal injection of chain urea with cephalosporins method, according to the random number table method they were divided into model group, apigenin group (50 mg/kg), ML385 group (Nrf2/HO-1 pathway inhibitor, dose of 20 mg/kg), apigenin + ML385 group (apigenin dose of 50 mg/kg, ML385 dose of 20 mg/kg), with 9 mice in each group. Another 9 mice were set as the control group. After grouping, the levels of fasting serum blood glucose (FSG), triglyceride (TG), total cholesterol (TC) and pathological changes of liver tissue were measured; the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were measured, and the levels of superoxide dismutase (SOD), malonic dialdehyde (MDA) and Nrf2/HO-1 pathway protein expression of rats in each group were detected. Results Compared with the control group, the liver tissue of the model group showed structural disorder, accompanied by inflammatory cell infiltration, hepatocyte degeneration, necrosis, cytoplasm contraction, deep staining and other pathological damage, the levels of FSG, TG, TC, serum TNF-α and IL-6, and MDA in liver tissue increased significantly (P ＜ 0.05), and SOD level, Nrf2, HO-1 protein expressions in liver tissue were significantly decreased (P ＜ 0.05). Compared with the model group, the pathological damage of liver tissue in ML385 group was aggravated, the levels of FSG, TG, TC, serum TNF-α and IL-6, and MDA in liver tissue increased significantly (P ＜ 0.05), and SOD level, Nrf2, HO-1 protein expressions in liver tissue were significantly decreased (P ＜ 0.05). Compared with the model group, the pathological damage of liver tissue in the apigenin group was alleviated, the levels of FSG, TG, TC, serum TNF-α and IL-6, and MDA in liver tissue decreased significantly (P ＜ 0.05), and SOD level, Nrf2 and HO-1 protein expressions in liver tissue were significantly increased (P ＜ 0.05). Compared with ML385 group, the pathological damage of liver tissue in apigenin+ML385 group was alleviated, the levels of FSG, TG, TC, serum TNF-α and IL-6, and MDA in liver tissue decreased significantly (P ＜ 0.05), and SOD level, Nrf2 and HO-1 protein expressions in liver tissue were significantly increased (P ＜ 0.05). Conclusion Apigenin can improve oxidative stress injury in gestational diabetic rats by activating Nrf2/HO-1 pathway.

Key words： Nrf2/HO-1 Apigenin Gestational diabetes mellitus Oxidative stress injury

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	YANG Li1 ZHAO Gang2 FAN Xiumei2 WU Lingling3 TIAN Shuqing1

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YANG Li1 ZHAO Gang2 FAN Xiumei2 WU Lingling3 TIAN Shuqing1. Study on apigenin improvement of oxidative stress injury in diabetic rats based on Nrf2/HO-1 pathway[J]. 中国医药导报, 2020, 17(13): 27-31.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I13/27

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