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| Study on the therapeutic effect and mechanism of Bairesi Pills on vitiligo model induced by hydroquinone in C57BL/6 mice |
| Reshalati·Yizize1 MA Li2 MA Fuchang2 HUO Shixia2 Silafu·Aibai2 LI Zhijian2 |
1.School of Traditional Uighur Medical, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China;
2.Department of Toxicology Laboratory, Xinjiang Institute of Traditional Uyghur Medicine, Xinjiang Uygur Autonomous Region, Urumqi 830049, China |
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Abstract Objective To investigate the therapeutic effect of Bairesi Pills on the skin coated with hydroquinone to establish a decolorized model of vitiligo in C57BL/6 mice, so as to provide experimental support for the preclinical study of Bairesi Pill. Methods The model of vitiligo in C57BL/6 mice was established by applying hydroquinone ointment on the skin: C57BL/6 mice, SPF level, 32 mice, male and female half. After passing quarantine, 2.5% hydroquinone ointment was applied on the skin for 20 days. All the model animals had vitiligo on their skin. According to the random number table of the size of vitiligo, the model animals were divided into the blank control group, the model control group, the Vitiligo Capsules group, and the Bairesi Pills treatment group, with 8 patients in each group, half male and half female. The blank control group and the model control group were given equal volume solvent according to 20 mL/kg, and the other groups were given corresponding solution once a day for 30 consecutive days. On the next day, the decolorization and decolorization area of the hair were observed and photographed. After the treatment, the eyeballs were collected and the blood was collected. The serum was isolated and the tyrosinase content was determined by enzyme-linked immunosorbent assay (ELISA). The hair follicles containing melanin were counted by hematoxylin-eosin (HE) staining in skin. Results There were statistically significant differences in the proportion of hair follicles, hair decolorization score and serum tyrosinase content in the four groups (all P ＜ 0.05). Compared with the blank control group, the proportion of melanin hair follicles and the serum tyrosinase content in the model control group decreased, and the hair decolorization score increased, with statistically significant differences (P ＜ 0.05 or P ＜ 0.01). Compared with the blank control group, the proportion of melanin hair follicles in the Vitiligo Capsules group and the Bairesi Pills treatment group decreased, and the hair decoloration score increased, the differences were highly statistically significant (all P ＜ 0.01), but there was no statistically significant difference in the serum tyrosinase content between the two groups compared with the blank control group (P ＞ 0.05). Compared with the model control group, the proportion of melanin hair follicle number, the serum tyrosinase content and the hair decolorization score in the Vitiligo Capsules group and the Bairesi Pills treatment group increased, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The proportion of melanin hair follicles, hair decolorization score and serum tyrosinase content in the Bairesi Pills treatment group were not statistically significant compared with the Vitiligo Capsules group (P ＞ 0.05). Conclusion Bairesi Pills can promote the increase of tyrosinase content, promote the proliferation of active melanocytes, so as to promote the generation of melanin, hair decolorization score is also significantly reduced, on the C57BL/6 mice model of vitiligo has a good therapeutic effect, and may have the same therapeutic effect as Vitiligo Capsules.
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[1] 石海霞，张汝芝.自噬在白癜风发生发展中的作用[J].国际皮肤性病学杂志，2017，43（5）：293-296.
[2] 毛秋霞，贾苇雪，肖学敏，等.节段型白癜风的研究进展[J].国际皮肤性病学杂志，2015，41（6）：388-391.
[3] Grimes PE，Miller MM. Vitiligo：Patient stories，self-esteem，and the psychological burden of disease [J]. Int J Womens Dermatol，2018，4（1）：32-37.
[4] 王春又，游弋，葛兰，等.白癜风免疫发病机制的研究进展[J].医学综述，2017，23（18）：3638-3641，3646.
[5] 米吉提·吾普尔，热孜万古丽·乌买尔.白癜风免疫机制研究进展[J].中国中西医结合皮肤性病学杂志，2016，15（2）：130-132.
[6] 刘哲，曲生明，巫毅，等.白癜风的免疫学发病机制及吡美莫司治疗白癜风的研究进展[J].中国老年学杂志，2017， 37（10）：2588-2592.
[7] 刘景卫.中药干预联合点阵激光对白癜风皮损特异性标记基因表达影响的相关研究[J].中医临床研究，2017，9（23）：13-15.
[8] 热孜万古丽·乌买尔，米吉提·吾普尔.结合维医理论论述白癜风病因与治疗研究进展[J].亚太传统医药，2017， 13（3）：16-18.
[9] 叶伊琳，唐洁，刘琴，等.复方补骨脂搽剂对豚鼠白癜风模型作用的实验研究[J].北京中医药大学学报，2014，37（4）：252-254，272，封3.
[10] Forman L. A nole on the depigmentary properties of monobenzyle-ther of hydroquinone [J]. Br J Dermatol，1953，65（11）：406-409.
[11] 杨欣.复方白芷酊治疗白癜风作用的实验及临床研究[D].武汉：湖北中医药大学，2015.
[12] 斯拉甫·艾白，玉素甫·买提努尔.白癜风的维吾尔医诊断与治疗[M].乌鲁木齐：新疆人民出版总社，2012：1.
[13] 王培杰，王璞.维医药“毒”涵义辨析[J].中医药导报，2017，23（10）：44-45.
[14] 斯拉甫·艾白，吐尔逊·乌甫尔，居来提·阿不都瓦衣提，等.基于维吾尔医理论关联分析白癜风辨证分型与成熟及清除疗法[J].新疆医学，2018，48（2）：139-142.
[15] 古再丽努尔，巴哈尔古丽，王兴伊.维吾尔医特色疗法治疗白癜风的研究概况[J].中国民族民间医药，2017， 26（20）：57-59，67.
[16] 米吉提·吾普尔，热孜万古丽·乌买尔，玉苏普·买提努尔，等.白癜风研究进展[J].中国民康医学，2018，30（15）：82-84，91.
[17] 沈丹蓓，孙建方.白癜风相关研究领域中常用的动物模型[J].外医学：皮肤性病学分册，2004，30（4）：226-228.
[18] 解翠林，石年，付曼妮，等.激光照射联合卤米松乳膏外用治疗白癜风的效果[J].中国医药导报，2018，15（34）：93-96.
[19] 陈美莲，林运霜，陈樱桃，等.辨证施护联合梅花针叩刺治疗白癜风的效果观察[J].中国医药科学，2019，9（3）：187-189.
[20] 章玲玲，许文，许爱娥.葛根素促进黑素细胞黑素合成及其机制的初步探讨[J].中华皮肤科杂志，2016，49（5）：338-341.
[21] 潘兴华，陈志龙，黄丽娜.黑素细胞及黑素的生成与调节[J].生理科学进展，1998（2）：85-57.
[22] 罗卫，蔡瑞康，朱美材，等.自体黑素细胞培养移植治疗白癜风的长期随访观察与研究[J].临床皮肤科杂志，2015，44（2）：78-80. |
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