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| Effect of salidroside/chitosan hydrogel on oxidative stress injury in rat adipose tissue derived mesenchymal stem cells |
| CHENG Yu ZHENG Jie CHEN Hao LI Jianlan HUANG Ling YAO Yaocong CHEN Can |
| Department of Cardiology, Affiliated Hospital of Guangdong Medical University, Guangdong Province, Zhanjiang 524000, China |
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Abstract Objective To investigate the effect of salidroside/chitosan hydrogel on oxidative stress injury in rat adipose tissue derived mesenchymal stem cells. Methods The cell lines were divided into Control group, H2O2 group (model group), low, medium and high concentration experimental group, while oxidative stress modeling was performed on the model group and each experimental group. The temperature sensitivity of the hydrogel was observed at different temperatures, the cell morphology was observed by a light microscope, the cell viability was detected by the CCK-8 method, while the cells were labeled with CM-Dil Dye for counting and 3D morphological observation. Results The hydrogel was in a sol state at 4℃ and in a gel state at 37℃. The cells in the medium concentration experimental group formed obvious colonies under the light microscope. There was no statistically significant difference in cell viability between the groups on days 0 and 1 (P ＞ 0.05). On days 3 and 7, the cell viability of the model group was lower than that of the Control group, while the cell viability of each experimental group was higher than that of the model group, and the differences were statistically significant (P ＜ 0.05). On days 0 and 1, there was no statistically significant difference in the number of cells in each group (P ＞ 0.05). On days 3 and 7, the number of cells in the model group was lower than that in the control group, while the number of cells in each experimental group was higher than that in the model group, and the differences were statistically significant (P ＜ 0.05). The 3D images observed that the cells in the medium concentration experimental group grew from spherical to dendritic with time. Conclusion Salidroside-modified hydrogel promotes survival of rat adipose stem cells injured by oxidative stress.
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