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| Study on plasma metabolomics of liver cancer patients with syndrome of dampness excessiveness due to spleen deficiency based on UPLC-HDMS |
| WANG Dong LI Jingjie LYU Shuqin |
| VIP Ward, Chinese Medicine Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830000, China |
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Abstract Objective To study the pathogenesis of liver cancer with syndrome of dampness excessiveness due to spleen deficiency by metabonomics and the molecular biological effect of the intervention of invigorating spleen for eliminating dampness. Methods Fifty persons with peace quality of blank group were selected from the physical examination center of Chinese Medicine Hospital of Xinjiang Medical University (hereinafter referred to as “our hospital”) from June 2017 to June 2019. 50 cases of liver cancer patients with spleen deficiency and dampness syndrome in our hospital were taken as control group and 50 cases of liver cancer patients taking Sijunzi Decoction for one month were taken as observation group. Metabonomics analysis of plasma samples from three groups of participants was carried out by using the technology of ultra-high performance liquid chromatography-high definition mass spectrometry. Results Nineteen potential biomarkers such as glutathione and L-glutamic acid were identified in the blood samples of the selected subjects. Compared with blank group, 6 of control group showed an upward trend and 13 showed a downward trend (P < 0.05 or P < 0.01). After intervention of Sijunzi Decoction, 17 of them had callback effect, and the adjustment difference was statistically significant (P < 0.05 or P < 0.01). According to these 17 callback biomarkers, 6 related metabolic pathways including D-glutamine and glutamate metabolism, taurine and taurine metabolism were found. Conclusion There are disorders of amino acid metabolism, arachidonic acid metabolism, fatty acid metabolism and glutathione metabolism in liver cancer of syndrome of dampness excessiveness due to spleen deficiency. Sijunzi Decoction may regulate the disorders by above metabolic pathways in liver cancer of syndrome of dampness excessiveness due to spleen deficiency.
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